Gene Expression Data
Assay Details
Assay
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Reference:
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J:72502
Perez-Armendariz EM, et al., Developmental regulation of connexin 43 expression in fetal mouse testicular cells. Anat Rec. 2001 Nov 1;264(3):237-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5640996
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Gene symbol:
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Gja1
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Gene name:
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gap junction protein, alpha 1
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Antibody:
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MAB3068
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Fixative was 70% ethanol.
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Results
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Specimen
4A:
embryonic day 12.5
(more )
Note: Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
Specimen
4A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
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Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
|
Specimen
4B:
embryonic day 14.5
(more )
Note: Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
Specimen
4B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: Leydig cell |
Leydig cell |
Present |
|
4B
|
|
Specimen
4C:
embryonic day 16.5
(more )
Note: Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
Specimen
4C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
|
Specimen
4D:
embryonic day 18.5
(more )
Note: Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
Specimen
4D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Hsd3b (assay MGI:5640782).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: Leydig cell |
Leydig cell |
Present |
|
4D
|
|
Specimen
5A:
embryonic day 14.5
(more )
Note: Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
Specimen
5A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: Sertoli cell |
Sertoli cell |
Present |
|
5A
|
Expression is in cells and at adjacent cell borders.
|
Specimen
5B:
embryonic day 16.5
(more )
Note: Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
Specimen
5B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: Sertoli cell |
Sertoli cell |
Present |
|
5B
|
Expression is in cells and at adjacent cell borders.
|
Specimen
5C:
embryonic day 18.5
(more )
Note: Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
Specimen
5C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: Sertoli cell |
Sertoli cell |
Present |
|
5C
|
Expression is in cells and at adjacent cell borders.
|
Specimen
5D:
embryonic day 18.5
(more )
Note: Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
Specimen
5D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; green - Amh (assay MGI:5640783).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: Sertoli cell |
Sertoli cell |
Present |
|
5D
|
Expression is in cells and at adjacent cell borders.
|
Specimen
6A:
embryonic day 11.5
(more )
Note: Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
Specimen
6A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
Regionally restricted |
6A
|
Expression is between Sertoli and germinal cells.
|
Specimen
6B:
embryonic day 12.5
(more )
Note: Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
Specimen
6B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primary sex cord |
Present |
|
6B
|
Expression is between Sertoli and germinal cells.
|
Specimen
6C:
embryonic day 14.5
(more )
Note: Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
Specimen
6C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: seminiferous cord |
Present |
|
6C
|
Expression is between Sertoli and germinal cells.
|
Specimen
6D:
embryonic day 16.5
(more )
Note: Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
Specimen
6D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: seminiferous cord |
Present |
Regionally restricted |
6D
|
Expression is between Sertoli and germinal cells.
|
Specimen
6E:
embryonic day 18.5
(more )
Note: Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
Specimen
6E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Gja1; blue - Gcna (assay MGI:5640997).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: seminiferous cord |
Present |
Regionally restricted |
6E
|
Expression is between Sertoli and germinal cells.
|
|