Hsd3b Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:72502 Perez-Armendariz EM, et al., Developmental regulation of connexin 43 expression in fetal mouse testicular cells. Anat Rec. 2001 Nov 1;264(3):237-46
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5640782
Gene symbol:	Hsd3b
Gene name:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase cluster

Antibody:	3bHSD antibody
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Fixative was 70% ethanol.

Results

Specimen 1C: embryonic day 12.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS20: Leydig cell	Leydig cell	Present		1C

Specimen 3A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Regionally restricted	3A	Expression is in cell clusters and in isolated cells.

Specimen 3B: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Leydig cell	Leydig cell	Present	Regionally restricted	3B	Expression is in cell clusters, particularly in the central part.

Specimen 3C: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Leydig cell	Leydig cell	Present		3C	Uniform distribution between the seminiferous cords.

Specimen 3D: embryonic day 18.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Leydig cell	Leydig cell	Present		3D	Uniform distribution between the seminiferous cords.

Specimen 4A: embryonic day 12.5 (more

)
Note: Double labeled: green - Hsd3b; red - Gja1 (assay MGI:5640996).

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Regionally restricted	4A	Expression is in cell clusters and in isolated cells.

Specimen 4B: embryonic day 14.5 (more

)
Note: Double labeled: green - Hsd3b; red - Gja1 (assay MGI:5640996).

Structure	Cell Type	Level	Pattern	Image	Note
TS22: Leydig cell	Leydig cell	Present		4B	Expression is in cell clusters, particularly in the central part.

Specimen 4C: embryonic day 16.5 (more

)
Note: Double labeled: green - Hsd3b; red - Gja1 (assay MGI:5640996).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: Leydig cell	Leydig cell	Present		4C	Uniform distribution between the seminiferous cords.

Specimen 4D: embryonic day 18.5 (more

)
Note: Double labeled: green - Hsd3b; red - Gja1 (assay MGI:5640996).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Leydig cell	Leydig cell	Present		4D	Uniform distribution between the seminiferous cords.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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