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Gene Expression Data
Assay Details
Assay
Reference: J:84056 Motoyama J, et al., Differential requirement for Gli2 and Gli3 in ventral neural cell fate specification. Dev Biol. 2003 Jul 1;259(1):150-61
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5575904
Gene symbol: Isl2
Gene name: insulin related protein 2 (islet 2)
Antibody: anti-Isl1/2
Detection system: Secondary antibody
Assay notes: The antibody used in this assay recognizes two related proteins.
Results
Specimen 1E: embryonic day 9.5 (more )
Note: This section is at the thoracic level. Double labeled: red - Isl1 and Isl2; black - Pax7 (assay MGI:5575400).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 1E Expression is in the motor neurons in the ventral neural tube.
Specimen 1F: embryonic day 9.5; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: This section is at the thoracic level. Double labeled: red - Isl1 and Isl2; black - Pax7 (assay MGI:5575400).
Structure Level Pattern Image Note
TS15: neural tube Present Ubiquitous 1F Expression is in the motor neurons.
Specimen 1G: embryonic day 9.5 (more )
Note: This section is at the level of the trunk. Double labeled: red - Isl1 and Isl2; black - Pax7 (assay MGI:5575400).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 1G Expression is in the motor neurons in the ventral neural tube.
Specimen 1H: embryonic day 9.5; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: This section is at the level of the trunk. Double labeled: red - Isl1 and Isl2; black - Pax7 (assay MGI:5575400).
Structure Level Pattern Image Note
TS15: neural tube Present Ubiquitous 1H Expression is in the motor neurons.
Specimen 1K: embryonic day 9.5 (more )
Note: Double labeled: green - Isl1 and Isl2; red - Foxa2 (assay MGI:5575402).
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 1K Expression is in the motor neurons in the ventral neural tube.
Specimen 1L: embryonic day 9.5; Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: Double labeled: green - Isl1 and Isl2; red - Foxa2 (assay MGI:5575402).
Structure Level Pattern Image Note
TS15: neural tube Present Ubiquitous 1L Expression is in the motor neurons.
Specimen 6F: embryonic day 9.5; Ptch1tm1Mps/Ptch1+ (more )
Note: This section is at the thoracic level.
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 6F Expression is ventral in motor neurons.
Specimen 6G: embryonic day 9.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J, Ptch1tm1Mps/Ptch1+ (more )
Note: This section is at the thoracic level.
Structure Level Pattern Image Note
TS15: neural tube Absent 6G
Specimen 6H: embryonic day 9.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3Xt-J, Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: This section is at the thoracic level.
Structure Level Pattern Image Note
TS15: neural tube Trace Regionally restricted 6H Expression is absent at this level (thoracic). The authors state that there were a few expressing cells at the trunk level.
Specimen 6I: embryonic day 9.5; Gli2tm1Alj/Gli2tm1Alj, Gli3Xt-J/Gli3+, Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: This section is at the thoracic level.
Structure Level Pattern Image Note
TS15: neural tube Weak Regionally restricted 6I Expression is ventral in motor neurons.
Specimen 6J: embryonic day 9.5; Gli2tm1Alj/Gli2tm1Alj, Ptch1tm1Mps/Ptch1tm1Mps (more )
Note: This section is at the thoracic level.
Structure Level Pattern Image Note
TS15: neural tube Present Regionally restricted 6J Expression is ventral in motor neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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