Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:157005
Park J, et al., Regulation of Sox9 by Sonic Hedgehog (Shh) is essential for patterning and formation of tracheal cartilage. Dev Dyn. 2010 Feb;239(2):514-26
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5573256
|
|
Gene symbol:
|
Sox9
|
|
Gene name:
|
SRY (sex determining region Y)-box 9
|
|
Antibody:
|
anti-Sox9
|
|
Detection system:
|
Secondary antibody
|
|
|
Results
|
Specimen
2A:
embryonic day 13.5
(more  )
Note: Double labeled: red - Sox9; green - BrdU.
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - BrdU.
|
Specimen
2B:
embryonic day 14.5
(more )
Note: Double labeled: red - Sox9; green - BrdU.
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - BrdU.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: trachea mesenchyme |
Strong |
Regionally restricted |
2B
|
Expression is ventral and is segmented.
|
Specimen
2C:
embryonic day 13.5; Shhtm1Amc/ Shhtm1Amc
(more )
Note: Double labeled: red - Sox9; green - BrdU.
Specimen
2C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - BrdU.
|
Specimen
2D:
embryonic day 14.5; Shhtm1Amc/ Shhtm1Amc
(more )
Note: Double labeled: red - Sox9; green - BrdU.
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - BrdU.
|
Specimen
4A:
embryonic day 11.5
(more )
Note: Double labeled: red - Sox9; green - BrdU.
Specimen
4A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - BrdU.
|
Specimen
4B:
embryonic day 11.5; Shhtm1Amc/ Shhtm1Amc
(more )
Note: Double labeled: red - Sox9; green - BrdU.
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - BrdU.
|
Specimen
4C:
embryonic day 11.5
(more )
Note: Double labeled: red - Sox9; green - TUNEL.
Specimen
4C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - TUNEL.
|
Specimen
4D:
embryonic day 11.5; Shhtm1Amc/ Shhtm1Amc
(more )
Note: Double labeled: red - Sox9; green - TUNEL.
Specimen
4D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sox9; green - TUNEL.
|
Specimen
5A:
embryonic day 13.5
(more )
Note: Also stained for Nkx2-1 (assay MGI:5573259).
Specimen
5A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Also stained for Nkx2-1 (assay MGI:5573259).
|
Specimen
5C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S1A:
embryonic day 17.5
(more )
Specimen
S1A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
S1B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools