Gene Expression Data
Assay Details
Assay
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Reference:
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J:183957
Wu F, et al., Onecut 1 and Onecut 2 are potential regulators of mouse retinal development. J Comp Neurol. 2012 Apr 1;520(5):952-69
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5509381
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Gene symbol:
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Atoh7
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Gene name:
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atonal bHLH transcription factor 7
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Reporter:
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HA
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Antibody:
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anti-HA tag (A00168)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Two anti-HA antibodies were described (goat polyclonal and rabbit polyclonal). The antibody used in the assays was not specified, so the goat polyclonal has been assigned. See the rabbit polyclonal at MGI:5509310.
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Results
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Specimen
7B,B',B''':
embryonic day 12.5; Atoh7tm1.1Xmu/?
(more )
Note: Double labeled: green - Atoh7; magenta - Pcna (assay MGI:5509378). This specimen could have been either heterozygous or homozygous for Math5HA.
Specimen
7B,B',B''':
(close )
Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Atoh7tm1.1Xmu/?
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Atoh7; magenta - Pcna (assay MGI:5509378). This specimen could have been either heterozygous or homozygous for Math5HA.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: retina |
Present |
Regionally restricted |
7B'
,
7B
,
7B'''
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Expression was detected largely in proliferating retinal progenitor cells.
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Specimen
7E,E',E''':
embryonic day 14.5; Atoh7tm1.1Xmu/?
(more )
Note: Double labeled: green - Atoh7; magenta - Pcna (assay MGI:5509378). This specimen could have been either heterozygous or homozygous for Math5HA.
Specimen
7E,E',E''':
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
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Mutant Allele(s):
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Atoh7tm1.1Xmu/?
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Atoh7; magenta - Pcna (assay MGI:5509378). This specimen could have been either heterozygous or homozygous for Math5HA.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: retina |
Present |
Regionally restricted |
7E
,
7E'
,
7E'''
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At E14.5, expression continued to be largely in proliferating cells.
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