Antibody:	Anti-P2X5
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS13: future brain	Present		2A , 2C	Very few positive cells colocalized with TUJ-1 positive cells.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2D , 2F	Expression was detected in the cortical plate. Most of the positive cells in the cortical plate colocalized with TUJ-1.

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Present	Uniform	3A , 3C	Colocalization with TUJ-1 was in the basal plate.
TS15: dorsal root ganglion	Present		3A , 3C

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	3D , 3F	Expression was detected in the ventral horn. Most of the positive cells colocalized with TUJ-1.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present	Uniform	4C , 4A	Expression colocalized with TUJ-1.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present	Uniform	4D , 4F	Expression colocalized with TUJ-1.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Strong		5A , 5C , 5D , 5F	Most positive cells colocalized with TUJ-1.
TS19: optic stalk	Strong	Regionally restricted	5A , 5D , 5F , 5C	Expression was detected in the optic nerve. Most positive cells colocalized with TUJ-1.

Structure	Level	Pattern	Image	Note
TS19: olfactory epithelium	Strong		6F , 6D , 6A , 6C

Structure	Level	Pattern	Image	Note
TS19: gut	Present		7C , 7A	All positive cells colocalized with TUJ-1.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	7F , 7D	Extensive labeling of nerve fibers was scattered throughout the skeletal muscle of the forelimb.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present
TS19: lung	Present			Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS21: brain	Moderate	Regionally restricted		Expression was detected in the neuroectoderm of brain vesicles.
TS21: cortical plate	Strong
TS21: optic nerve	Strong
TS21: retina	Strong
TS21: spinal cord	Present	Non-Uniform		Expression was strong in the ventral horn and weak in the dorsal horn of the spinal cord.
TS21: blood vessel	Moderate
TS21: olfactory epithelium	Moderate
TS21: gut	Moderate			Expression was in the neurons.
TS21: lung	Moderate			Expression was in the neurons.
TS21: skeletal muscle	Moderate			Expression was in the nerve fiber.

Structure	Level	Pattern	Image	Note
TS25: brain	Weak	Regionally restricted		Expression was detected in the neuroectoderm of brain vesicles.
TS25: cortical plate	Moderate
TS25: dorsal root ganglion	Moderate
TS25: ventral grey horn	Moderate
TS25: dorsal grey horn	Absent
TS25: blood vessel	Weak
TS25: olfactory epithelium	Weak
TS25: optic nerve	Weak
TS25: retina	Weak
TS25: gut	Weak			Expression was in neurons.
TS25: lung	Weak			Expression was in neurons.
TS25: skeletal muscle	Weak			Expression was in nerve fiber.