Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51099
Asakura A, et al., Apoptosis of epaxial myotome in Danforth's short-tail (Sd) mice in somites that form following notochord degeneration. Dev Biol. 1998 Nov 15;203(2):276-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5489064
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe17
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Embryos were fixed in glutaraldehyde/paraformaldehyde (0.2%/0.4%).
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Results
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Specimen
2A:
embryonic day 10.0
(more  )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
2A:
(close  )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
2A
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Expression in entire myotome from certical to lumbar region.
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| TS16: somite |
Present |
Regionally restricted |
2A
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Expression in in the dorsomedial lip of newly formed somites in the sacral region.
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Specimen
2B:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Regionally restricted |
2B
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Expression in entire myotome in cervical and thoracic regions; reduced in dorsomedial myotome in lumbar and sacral regions.
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Specimen
2C:
embryonic day 10.0
(more )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
2C:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
|
|
Note:
|
Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
2C
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Expression in entire myotome from certical to lumbar region.
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| TS16: somite |
Present |
Regionally restricted |
2C
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Expression in in the dorsomedial lip of newly formed somites in the sacral region.
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Specimen
2D:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
Other - See Notes
|
|
Note:
|
Also stained for Shh (assay MGI:5489063).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Regionally restricted |
2D
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Expression in entire myotome in cervical and thoracic region.
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| TS16: somite |
Present |
Regionally restricted |
2D
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In newly formed somites of sacral region, expression expanded more ventrally than normal.
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Specimen
2E:
embryonic day 10.0
(more )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
2E:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
2E
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Expression in entire myotome from certical to lumbar region.
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| TS16: somite |
Strong |
Regionally restricted |
2E
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Expression in in the dorsomedial lip of newly formed somites in the sacral region.
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Specimen
2F:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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|
Embedding:
|
Gelatin
|
|
Note:
|
Also stained for Shh (assay MGI:5489063).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Regionally restricted |
2F
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Expression in entire myotome in cervical and thoracic region.
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| TS16: somite |
Present |
Regionally restricted |
2F
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In newly formed somites of sacral region, expression expanded more ventrally than normal.
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Specimen
2G:
embryonic day 10.0
(more )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
2G:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
|
Gelatin
|
|
Note:
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Also stained for Shh (assay MGI:5489063).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
2G
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Expression in entire myotome from certical to lumbar region.
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| TS16: somite |
Present |
Regionally restricted |
2G
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Expression in in the dorsomedial lip of newly formed somites in the sacral region.
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Specimen
2H:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
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Gelatin
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Note:
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Also stained for Shh (assay MGI:5489063).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk myotome |
Weak |
Regionally restricted |
2H
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Expression reduced in dorsomedial myotome in lumbar and sacral regions.
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Specimen
2I:
embryonic day 10.0
(more )
Note: Also stained for Shh (assay MGI:5489063).
Specimen
2I:
(close )
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Genetic Background:
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involves: RSV
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Age:
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embryonic day 10.0
|
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Sex:
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Not Specified
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Type:
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section from whole mount
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|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Gelatin
|
|
Note:
|
Also stained for Shh (assay MGI:5489063).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Widespread |
2I
|
Expression in entire myotome from certical to lumbar region.
|
| TS16: somite |
Present |
Regionally restricted |
2I
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Expression in in the dorsomedial lip of newly formed somites in the sacral region.
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Specimen
2J:
(close )
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Genetic Background:
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involves: Danforth's duplication stock * RSV
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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Other - See Notes
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Embedding:
|
Gelatin
|
|
Note:
|
Also stained for Shh (assay MGI:5489063).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: myotome |
Present |
Regionally restricted |
2J
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Expression in entire myotome in cervical and thoracic regions.
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