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Gene Expression Data
Assay Details
Assay
Reference: J:51099 Asakura A, et al., Apoptosis of epaxial myotome in Danforth's short-tail (Sd) mice in somites that form following notochord degeneration. Dev Biol. 1998 Nov 15;203(2):276-89
Assay type: RNA in situ
MGI Accession ID: MGI:5489063
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe81
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Embryos were fixed in glutaraldehyde/paraformaldehyde (0.2%/0.4%).
Results
Specimen 1A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube floor plate Present Widespread 1A Expression in ventral axial structures, notochord and floorplate, extending rostrally to caudally.
TS17: notochord Present Widespread 1A Expression in ventral axial structures, notochord and floorplate, extending rostrally to caudally.
Specimen 1B: embryonic day 10.5; Etn2Sd/Etn2Sd (more )
Structure Level Pattern Image Note
TS17: neural tube floor plate Present Regionally restricted 1B Expression in the rostral (cervical and thoracic) floorplate. Expression not detected in axial structures in the caudal (lumbosacral) region or in the region where the notochord had degenerated.
Specimen 2A: embryonic day 10.0 (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: future brain Present 2A
TS16: forelimb bud Present Regionally restricted 2A Expression in posterior mesenchyme of limb buds.
TS16: hindlimb bud Present Regionally restricted 2A Expression in posterior mesenchyme of limb buds.
Specimen 2B: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: future brain Present 2B
TS16: forelimb bud Present Regionally restricted 2B Expression in posterior mesenchyme of limb buds.
TS16: hindlimb bud Present Regionally restricted 2B Expression in posterior mesenchyme of limb buds.
Specimen 2C: embryonic day 10.0 (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: hindgut Present 2C
TS16: forelimb bud Present Regionally restricted 2C Expression in posterior mesenchyme of limb buds.
TS16: hindlimb bud Present Regionally restricted 2C Expression in posterior mesenchyme of limb buds.
Specimen 2D: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: hindgut Present 2D
TS16: forelimb bud Present Regionally restricted 2D Expression in posterior mesenchyme of limb buds.
TS16: hindlimb bud Present Regionally restricted 2D Expression in posterior mesenchyme of limb buds.
TS16: neural tube floor plate Present Regionally restricted 2D Expression in cervical and thoracic floorplate; not detected in caudal floorplate.
TS16: notochord Absent 2D
Specimen 2E: embryonic day 10.0 (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present 2E
TS16: notochord Present 2E
Specimen 2F: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present Regionally restricted 2F Expression not detected in caudal floorplate region.
TS16: notochord Absent 2F
Specimen 2G: embryonic day 10.0 (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present 2G
TS16: notochord Present 2G
Specimen 2H: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present Regionally restricted 2H Expression not detected in caudal floorplate region.
TS16: notochord Absent 2H
Specimen 2I: embryonic day 10.0 (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: neural tube Present 2I
TS16: neural tube floor plate Present 2I
Specimen 2J: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myf5 (assay MGI:5489064).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present Regionally restricted 2J Expression in cervical and thoracic floorplate.
TS16: notochord Absent 2J
Specimen 3A: embryonic day 10.0 (more )
Note: Also stained for Pax1 (assay MGI:5489065).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present 3A
TS16: notochord Present 3A
Specimen 3B: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Pax1 (assay MGI:5489065).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present Regionally restricted 3B Expression absent in caudal floorplate.
TS16: notochord Absent 3B
Specimen 3C: embryonic day 10.0 (more )
Note: Also stained for Pax1 (assay MGI:5489065).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present 3C
TS16: notochord Present 3C
Specimen 3D: embryonic day 10.0; Etn2Sd/Etn2Sd (more )
Note: Also stained for Pax1 (assay MGI:5489065).
Structure Level Pattern Image Note
TS16: neural tube floor plate Present Regionally restricted 3D Expression absent in caudal floorplate.
TS16: notochord Absent 3D
Specimen 5A: embryonic day 10.5 (more )
Note: Also stained for Myog (assay MGI:5489067).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 5A
TS17: notochord Present 5A
Specimen 5B: embryonic day 10.5; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myog (assay MGI:5489067).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present Regionally restricted 5B No expression in caudal floorplate region.
TS17: notochord Absent 5B
Specimen 5C: embryonic day 10.5 (more )
Note: Also stained for Myod1 (assay MGI:5489068).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 5C
TS17: notochord Present 5C
Specimen 5D: embryonic day 10.5; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myod1 (assay MGI:5489068).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present Regionally restricted 5D No expression in caudal floorplate region.
TS17: notochord Absent 5D
Specimen 5E: embryonic day 10.5 (more )
Note: Also stained for Myod1 (assay MGI:5489068).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 5E
TS17: notochord Present 5E
Specimen 5F: embryonic day 10.5; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myod1 (assay MGI:5489068).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present Regionally restricted 5F No expression in caudal floorplate region.
TS17: notochord Absent 5F
Specimen 5G: embryonic day 10.5 (more )
Note: Also stained for Myod1 (assay MGI:5489068).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 5G
Specimen 5H: embryonic day 10.5; Etn2Sd/Etn2Sd (more )
Note: Also stained for Myod1 (assay MGI:5489068).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present Regionally restricted 5H No expression in caudal floorplate region.
TS17: notochord Absent 5H
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory