Probe:	D1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: conceptus	Absent		1A

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	1B	Expression was detected in the epiblast.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	1C	Expression was detected in the epiblast.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	1D	Expression increased in the epiblast compared to E6.5.
TS9: embryo mesoderm	Present	Regionally restricted	1D	Expression was detected in mesoderm emerging from the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	1E	Expression increased in the epiblast compared to E6.5.
TS10: embryo mesoderm	Present	Regionally restricted	1E	Expression was detected in mesoderm emerging from the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: amniotic fold	Present		1F
TS10: embryo	Present	Regionally restricted	1F	Expression was detected in the head process at the distal tip of the embryo.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Uniform	1G
TS10: embryo mesoderm	Present	Uniform	1G
TS10: embryo endoderm	Absent		1G
TS10: yolk sac mesoderm	Absent		1G
TS10: allantois	Present		1G
TS10: amniotic fold	Present		1G

Structure	Level	Pattern	Image	Note
TS10: allantois	Present		1H

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Uniform	1I
TS10: embryo mesoderm	Present	Uniform	1I
TS10: embryo endoderm	Absent		1I

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1J
TS11: amnion	Absent		1J

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Widespread	4A
TS12: yolk sac	Absent		4A

Structure	Level	Pattern	Image	Note
TS12: head	Present		4B
TS12: primitive streak	Present	Regionally restricted	4B	Expression was detected in the caudal-most primitive streak region.

Structure	Level	Pattern	Image	Note
TS12: head	Present		4C
TS12: somite	Present		4C
TS12: primitive streak	Present	Widespread	4C	Expression encompassed the whole region of the regressing primitive streak.
TS12: neural ectoderm	Present		4C
TS12: neural tube	Present		4C

Structure	Level	Pattern	Image	Note
TS12: head	Present		4D
TS12: neural ectoderm	Present		4D
TS12: neural tube	Present		4D
TS12: somite	Present		4D
TS12: primitive streak	Present	Widespread	4D	Expression encompassed the whole region of the regressing primitive streak.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Strong	Regionally restricted	4E	Expression was detected in the ventral midline of the forebrain.
TS12: future midbrain	Strong		4E

Structure	Level	Pattern	Image	Note
TS12: rhombomere	Present	Regionally restricted	4F	Expression was detected in presumptive rhombomere 4.
TS12: rhombomere floor plate	Weak	Regionally restricted	4F	Expression in presumptive rhombomere 4 was reduced in the floorplate.
TS12: neural groove	Weak	Regionally restricted	4F	Expression was reduced in the dorsal tips of the neural groove.

Structure	Level	Pattern	Image	Note
TS12: neural fold	Present	Regionally restricted	4G	Expression was progressively restricted to the dorsal side of the closing neural tube.
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	4G	Expression was detected in foregut endoderm in the region of the pharyngeal pouches.

Structure	Level	Pattern	Image	Note
TS12: neural tube	Ambiguous		4H	Expression was uniformly downregulated in the neural tube after closure.
TS12: somite	Strong		4H

Structure	Level	Pattern	Image	Note
TS12: neural groove	Strong	Regionally restricted	4I	Expression was detected in the caudal region of the neural groove.
TS12: splanchnopleure	Strong		4I

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present
TS12: amnion ectoderm	Absent
TS12: amnion mesenchyme	Absent
TS12: blood island	Absent
TS12: cardiac muscle tissue	Absent
TS12: endocardial tube	Absent
TS12: somatopleure	Absent
TS12: surface ectoderm	Absent
TS12: yolk sac endoderm	Absent
TS12: yolk sac mesenchyme	Absent
TS12: foregut diverticulum endoderm	Strong
TS12: hindgut diverticulum endoderm	Strong
TS12: paraxial mesenchyme	Strong
TS12: somite	Strong
TS12: splanchnopleure	Strong
TS12: neural ectoderm	Strong			Expression was strong in the neural ectoderm in the anterior region of the embryo. Expression level varied in the posterior region of the embryo.
TS12: neural tube	Ambiguous
TS12: allantois	Present	Regionally restricted		Expression was detected in the base of the allantois.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		7A

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		7A inset

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Strong		7B
TS13: rhombomere	Strong	Regionally restricted	7B	A high expression level was detected in rhombomeres 6 and 7.
TS13: rhombomere 2	Weak		7B
TS13: rhombomere 3	Weak		7B
TS13: rhombomere 5	Weak		7B

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Strong		7C
TS13: rhombomere	Strong	Regionally restricted	7C	A high expression level was detected in rhombomeres 6 and 7.
TS13: rhombomere 2	Weak		7C
TS13: rhombomere 3	Weak		7C
TS13: rhombomere 5	Weak		7C

Structure	Level	Pattern	Image	Note
TS15: rhombomere 3	Trace		7D
TS15: rhombomere 5	Trace		7D

Structure	Level	Pattern	Image	Note
TS15: rhombomere 3	Trace		7E
TS15: rhombomere 5	Trace		7E