Antibody:	Anti-Sox9 (AB5535)
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Strong	Regionally restricted	1B , 1C	Prominent expression in the entire otic epithelium, stronger on the medial side of the otic vesicle.
TS15: otocyst mesenchyme	Weak	Regionally restricted	1C , 1B	Faint expression in the periotic mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2A' , 2A"	Expression in the otic epithelium and periotic mesenchyme, with regions of relatively stronger and weaker expression.
TS17: otocyst mesenchyme	Present	Regionally restricted	2A' , 2A"	Expression in the otic epithelium and periotic mesenchyme, with regions of relatively stronger and weaker expression.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2B' , 2B"	Expression in the otic epithelium and periotic mesenchyme, with regions of relatively stronger and weaker expression.
TS17: otocyst mesenchyme	Present	Regionally restricted	2B' , 2B"	Expression in the otic epithelium and periotic mesenchyme, with regions of relatively stronger and weaker expression.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2C" , 2C'	Expression in the otic epithelium and periotic mesenchyme, with regions of relatively stronger and weaker expression.
TS17: otocyst mesenchyme	Present	Regionally restricted	2C" , 2C'	Expression in the otic epithelium and periotic mesenchyme, with regions of relatively stronger and weaker expression.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	3A" , 3A'	Expression in periotic mesenchyme and otic epithelium.
TS20: inner ear mesenchyme	Present	Regionally restricted	3A" , 3A'	Expression in periotic mesenchyme and otic epithelium.
TS20: semicircular canal	Present	Regionally restricted	3A' , 3A"	In lateral crista, relatively weaker expression closer to the dorsal boundary.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	3B' , 3B"	Expression in periotic mesenchyme and otic epithelium.
TS20: inner ear mesenchyme	Present	Regionally restricted	3B' , 3B"	Expression in periotic mesenchyme and otic epithelium.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	3C" , 3C'	Expression in periotic mesenchyme and otic epithelium.
TS20: inner ear mesenchyme	Present	Regionally restricted	3C" , 3C'	Expression in periotic mesenchyme and otic epithelium.
TS20: cochlear duct	Present	Regionally restricted	3C' , 3C"	Relatively weaker expression in regions of the cochlear duct.`

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present		3D	Expression in periotic mesenchyme and otic epithelium.
TS20: inner ear mesenchyme	Present		3D	Expression in periotic mesenchyme and otic epithelium.
TS20: utricle	Present	Regionally restricted	3D	Expression in the utricular macula limited to supporting cells.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Present	Regionally restricted	3E	Expression in periotic mesenchyme and otic epithelium.
TS20: inner ear mesenchyme	Present	Regionally restricted	3E	Expression in periotic mesenchyme and otic epithelium.
TS20: semicircular canal	Present	Regionally restricted	3E	Relatively weaker expression in regions of the posterior crista.

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	4A' , 4A"	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: inner ear mesenchyme	Present	Regionally restricted	4A' , 4A"	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: semicircular canal	Present	Regionally restricted	4A" , 4A'	Expression in supporting cells of the anterior and lateral cristae.

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	4B" , 4B'	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: inner ear mesenchyme	Present	Regionally restricted	4B" , 4B'	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: semicircular canal	Present	Regionally restricted	4B" , 4B'	Expression in supporting cells, but not in hair cells, of the posterior crista.

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	4C	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: inner ear mesenchyme	Present	Regionally restricted	4C	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: utricle	Present	Regionally restricted	4C	Expression in supporting cells, but not in hair cells, of the utricular macula.

Structure	Level	Pattern	Image	Note
TS22: inner ear epithelium	Present	Regionally restricted	4D	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: inner ear mesenchyme	Present	Regionally restricted	4D	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: saccule	Present	Regionally restricted	4D	Expression in supporting cells, but not in hair cells, of the posterior saccular macula.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Present	Regionally restricted	4E	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: cochlear ganglion	Absent		4E	No expression in the less differentiated spiral ganglion.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Present	Regionally restricted	4F	Expression in periotic mesenchyme and most of the otic epithelium.
TS22: cochlear ganglion	Absent		4F	No expression in the less differentiated spiral ganglion.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	5A	Expression in the cochlear duct, initially in the prosensory epithelium.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	5B

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	5C	Expression in the cochlear duct in more differentiated sensory epithelium at the basal end restricted to supporting cells.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct	Present	Regionally restricted	5D	Expression in the cochlear duct in more differentiated sensory epithelium at the basal end restricted to supporting cells.

Structure	Level	Pattern	Image	Note
TS24: vestibulo-cochlear ganglion	Present	Regionally restricted	5E
TS24: cochlear ganglion	Absent		5E	No expression in less differentiated spiral ganglion cells.

Structure	Level	Pattern	Image	Note
TS24: cochlear ganglion	Present		5F	Expression in more differentiated spiral ganglion cells.

Structure	Level	Pattern	Image	Note
TS24: cochlear ganglion	Present		5G	Expression in the spiral ganglion.

Structure	Level	Pattern	Image	Note
TS24: cochlear ganglion	Present	Regionally restricted	5H	Expression in more differentiated spiral ganglion cells.

Structure	Level	Pattern	Image	Note
TS24: saccule	Present	Regionally restricted	5I	Expression in saccular macula, only in supporting cells; no expression in hair cells.

Structure	Level	Pattern	Image	Note
TS24: utricle	Present	Regionally restricted	5J	Expression in utricular macula, only in supporting cells; no expression in hair cells.

Structure	Level	Pattern	Image	Note
TS24: semicircular canal	Present	Regionally restricted	5K' , 5K"	Expression in anterior and lateral crista, only in supporting cells; no expression in hair cells.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present	Regionally restricted	6A	In the undifferentiated cochlear duct, expression throughout the otic epithelium.

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present		6B

Structure	Level	Pattern	Image	Note
TS26: cochlear duct	Present	Regionally restricted	6C	Expression restricted to supporting cells, including inner and outer pillar cells, inner phalangeal supporting cells and Deiters' cells.

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present	Regionally restricted	6D	Dispersed expression in the spiral ganglion int in discretely positive cells of non-neuronal identity.

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present	Regionally restricted	6E	Dispersed expression in the spiral ganglion in discretely positive cells of non-neuronal identity.

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present		6F	Dispersed expression in the spiral ganglion int in discretely positive cells of non-neuronal identity.

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present	Regionally restricted	6G	Dispersed expression in the spiral ganglion int in discretely positive cells of non-neuronal identity.

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present	Regionally restricted	6H	Dispersed expression in the spiral ganglion int in discretely positive cells of non-neuronal identity.

Structure	Level	Pattern	Image	Note
TS26: semicircular canal	Present	Regionally restricted	6I	In the anterior crista, expression in supporting cells, but not in hair cells.

Structure	Level	Pattern	Image	Note
TS26: semicircular canal	Present	Regionally restricted	6J	In the lateral crista, expression in supporting cells, but not in hair cells.

Structure	Level	Pattern	Image	Note
TS26: utricle	Present	Regionally restricted	6K	In the utricular macula, expression in supporting cells, but not in hair cells.

Structure	Level	Pattern	Image	Note
TS26: saccule	Present	Regionally restricted	6L	In the saccular macula, expression in supporting cells, but not in hair cells.