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Gene Expression Data
Assay Details
Assay
Reference: J:150717 Cantagrel V, et al., Spatiotemporal expression in mouse brain of Kiaa2022, a gene disrupted in two patients with severe mental retardation. Gene Expr Patterns. 2009 Sep;9(6):423-9
Assay type: RNA in situ
MGI Accession ID: MGI:4353150
Gene symbol: Nexmif
Gene name: neurite extension and migration factor
Probe: Kiaa2022 cDNA1
Probe preparation: labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: brain mantle layer Present Regionally restricted 2A Expression is in post-mitotic cells.
Specimen 2B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: brain mantle layer Present Regionally restricted 2B Expression is in post-mitotic cells.
TS19: diencephalon lateral wall mantle layer Strong 2B
Specimen 2C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: brain mantle layer Present Regionally restricted 2C Expression is in post-mitotic cells.
TS19: neural tube mantle layer Strong 2C
Specimen 2D: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: brain mantle layer Present Regionally restricted 2D Expression is in post-mitotic cells.
Specimen 2E: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thalamus Strong Regionally restricted 2E Expression is in the dorsal thalamus.
TS20: hypothalamus Strong Regionally restricted 2E Expression is in the preoptic and tuberal hypothalamus.
TS20: midbrain Strong Regionally restricted 2E Expression is in the tegmentum.
TS20: hindbrain Strong Regionally restricted 2E Expression is in the ventral rhombencephalon.
Specimen 2F: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain Strong Regionally restricted 2F Expression is in the tegmentum.
TS20: diencephalon Strong 2F
Specimen 2G: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thalamus Strong Regionally restricted 2G
Specimen 2H: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: thalamus Strong Regionally restricted 2H
Specimen 2I: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Present Regionally restricted 2I Expression is in the preplate and neopallial cortex.
TS21: tongue Weak 2I
TS21: forebrain ventricular layer Absent 2I
TS21: hindbrain ventricular layer Absent 2I
TS21: midbrain ventricular layer Absent 2I
TS21: spinal cord ventricular layer Absent 2I
Specimen 2J: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Present Regionally restricted 2J Expression is in the preplate and neopallial cortex.
TS21: forebrain ventricular layer Absent 2J
TS21: hindbrain ventricular layer Absent 2J
TS21: midbrain ventricular layer Absent 2J
TS21: spinal cord ventricular layer Absent 2J
Specimen 2K: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Present Regionally restricted 2K Expression is in the preplate and neopallial cortex.
TS21: medulla oblongata Present 2K
TS21: forebrain ventricular layer Absent 2K
TS21: hindbrain ventricular layer Absent 2K
TS21: midbrain ventricular layer Absent 2K
TS21: spinal cord ventricular layer Absent 2K
Specimen 2L: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Present Regionally restricted 2L Expression is in the preplate and neopallial cortex.
TS21: forebrain ventricular layer Absent 2L
TS21: hindbrain ventricular layer Absent 2L
TS21: midbrain ventricular layer Absent 2L
TS21: spinal cord ventricular layer Absent 2L
Specimen Not shown E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: lobar bronchus Weak
Specimen 3A: postnatal day 3 (more )
Structure Level Pattern Image Note
TS27: cerebellum Present 3A
TS27: cerebral cortex Present 3A
TS27: main olfactory bulb Present 3A
TS27: entorhinal cortex Strong 3A
TS27: piriform cortex Strong 3A
TS27: hippocampus Strong Regionally restricted 3A Expression is in the pyramidal layer.
Specimen 3B,C,D: postnatal day 3 (more )
Note: C,D: Higher magnifications of boxed areas in B.
Structure Level Pattern Image Note
TS27: cerebral cortex Present 3C , 3B
TS27: dentate gyrus Present Regionally restricted 3B Expression is in the hippocampal fissure.
TS27: hippocampus Present Regionally restricted 3B Expression is in the hippocampal fissure.
TS27: mammillary body Strong Regionally restricted 3B , 3D Expression is in the hypothalamic ventral premammillary nucleus.
TS27: entorhinal cortex Strong 3B
Specimen 3G: postnatal day 45 (more )
Structure Level Pattern Image Note
TS28: cerebellum Weak 3G
TS28: cerebral cortex Weak 3G
TS28: hippocampus Weak 3G
TS28: main olfactory bulb Weak 3G
Specimen 3H,I,J: postnatal day 45 (more )
Note: I,J: Higher magnification of boxed areas in H.
Structure Level Pattern Image Note
TS28: cerebral cortex Weak 3H , 3I
TS28: hippocampus Weak 3H , 3I
TS28: mammillary body Weak Regionally restricted 3H , 3J Expression is in the hypothalamic ventral premammillary nucleus.
Specimen Not shown P20: postnatal day 20 (more )
Structure Level Pattern Image Note
TS28: mammillary body Moderate Regionally restricted Expression is in the hypothalamic ventral premammillary nucleus.
Specimen 4A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: midbrain mantle layer Present 4A
Specimen 4C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hypothalamus Present 4C
Specimen 4E: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: diencephalon Present 4E
TS20: hindbrain Present 4E
TS20: telencephalon Present 4E
TS20: brain ventricular layer Absent 4E
Specimen 5A,B: embryonic day 12.5 (more )
Note: Double labeled: purple - Nexmif; red - Tubb3 (assay MGI:4353151).
Structure Level Pattern Image Note
TS20: hypothalamus Present 5B , 5A
Specimen 5D,E: embryonic day 12.5 (more )
Note: Double labeled: purple - Nexmif; green - Th (assay MGI:4353796).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5E , 5D Expression is in the tegmentum.
Specimen 5G: postnatal day 3 (more )
Note: Double labeled: purple - Nexmif; green - Rbfox3 (assay MGI:4353797).
Structure Level Pattern Image Note
TS27: entorhinal cortex Present 5G
Specimen 5I,J,L: postnatal day 3 (more )
Note: Double labeled: black - Nexmif; green - Rbfox3 (assay MGI:4353797).
Structure Level Pattern Image Note
TS27: mammillary body Present Regionally restricted 5I , 5J , 5L Expression is in the hypothalamic ventral premammillary nucleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory