Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3776083
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Gene symbol:
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Met
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Gene name:
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met proto-oncogene
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Probe:
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Met probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5F:
embryonic day 11.5
(more  )
Note: 52 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
Specimen
5F:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S2/SvPas * CD-1
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Mutant Allele(s):
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Gdnftm1Rosl/Gdnftm1Rosl
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
5G right
,
5G left
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Expression in a portion of the C7-C8 region of the spinal cord was severely reduced but was unaffected outside this domain.
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Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4tm1Arbr
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
5H left
,
5H right
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Expression in a portion of the C7-C8 region of the spinal cord was severely reduced but was unaffected outside this domain.
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Specimen
6A:
embryonic day 11.0
(more )
Note: 41 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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41 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
6A left
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6A right
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Expression was detected in medial motor column motor neurons, and most of the lateral motor column in the C5-C8 spinal segments was largely devoid of expression.
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Specimen
6B:
embryonic day 11.0
(more )
Note: 44-45 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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44-45 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
6B left
,
6B right
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Expression was detected in a few neurons in the C8 region of the spinal cord.
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Specimen
6C:
embryonic day 11.5
(more )
Note: 47-48 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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47-48 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
6C right
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6C left
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
6D:
embryonic day 11.5
(more )
Note: 52 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
Regionally restricted |
6D left
,
6D right
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
S2A:
embryonic day 12.5
(more )
Specimen
S2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
S2A
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
S2D:
embryonic day 12.5
(more )
Specimen
S2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
S2D
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
S2F:
embryonic day 12.5
(more )
Note: Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
Specimen
S2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Met; red - Etv4 (assay MGI:3776080).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
S2F
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Expression was detected in the C7 region of the spinal cord.
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