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Gene Expression Data
Assay Details
Assay
Reference: J:73282 Watari N, et al., Hoxa3 regulates integration of glossopharyngeal nerve precursor cells. Dev Biol. 2001 Dec 1;240(1):15-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2156493
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-NF160
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3B,C: embryonic day 11.5 (more )
Note: Double labeled: green - Nefm; red - Isl1 (assay MGI:2156495).
Structure Level Pattern Image Note
TS19: glossopharyngeal ganglion Present Uniform 3C , 3B
TS19: vagus ganglion Present Uniform 3C , 3B
Specimen 3E,F: embryonic day 11.5; Hoxa3tm1Mrc/Hoxa3tm1Mrc (more )
Note: Type 1 mutant. Double labeled: green - Nefm; red - Isl1 (assay MGI:2156495).
Structure Level Pattern Image Note
TS19: glossopharyngeal ganglion Present Uniform 3F , 3E The ganglia of the IXth nerve are present, but they do not form an isolated ganglion from the Xth nerve proximal ganglion.
TS19: vagus ganglion Present Uniform 3F , 3E The ganglia of the IXth nerve are present, but they do not form an isolated ganglion from the Xth nerve proximal ganglion.
Specimen 3H,I: embryonic day 11.5; Hoxa3tm1Mrc/Hoxa3tm1Mrc (more )
Note: Type 2 mutant. Double labeled: green - Nefm; red - Isl1 (assay MGI:2156495).
Structure Level Pattern Image Note
TS19: glossopharyngeal ganglion Present Uniform 3H , 3I The ganglia of the IXth nerve are present, but they do not form an isolated ganglion from the Xth nerve proximal ganglion.
TS19: vagus ganglion Present Uniform 3H , 3I The ganglia of the IXth nerve are present, but they do not form an isolated ganglion from the Xth nerve proximal ganglion.
Specimen 5B,C: embryonic day 10.25 (more )
Note: Double labeled: green - Nefm; red - Isl1 (assay MGI:2156495).
Structure Level Pattern Image Note
TS17: glossopharyngeal ganglion Present Regionally restricted 5C , 5B The IXth nerve placode -derived cells are distributed in a stream from beneath the placode to where the proximal ganglion neurons will aggregate.
TS17: vagus ganglion Present Regionally restricted 5C , 5B The IXth nerve placode -derived cells are distributed in a stream from beneath the placode to where the proximal ganglion neurons will aggregate.
Specimen 5E,F: embryonic day 10.25; Hoxa3tm1Mrc/Hoxa3tm1Mrc (more )
Note: Type 1 mutant. Double labeled: green - Nefm; red - Isl1 (assay MGI:2156495).
Structure Level Pattern Image Note
TS17: glossopharyngeal ganglion Present Regionally restricted 5F , 5E No signal is detected near the proximal ganglion-forming region.
Specimen 5H,I: embryonic day 10.25; Hoxa3tm1Mrc/Hoxa3tm1Mrc (more )
Note: Type 2 mutant. Double labeled: green - Nefm; red - Isl1 (assay MGI:2156495).
Structure Level Pattern Image Note
TS17: glossopharyngeal ganglion Present Regionally restricted 5I , 5H In these abnormal embryos there is signal that connects between the placode-derived populations and the neural tube.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
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