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Gene Expression Data
Assay Details
Assay
Reference: J:58081 Lettice L, et al., The dominant hemimelia mutation uncouples epithelial-mesenchymal interactions and disrupts anterior mesenchyme formation in mouse hindlimbs. Development. 1999 Nov;126(21):4729-36
Assay type: RNA in situ
MGI Accession ID: MGI:1349142
Gene symbol: Fgf8
Gene name: fibroblast growth factor 8
Probe: Fgf8 probe12
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 3D +/+: embryonic day 11.5 (more )
Note: Double labeled: purple - Fgf8; blue - Shh (assay MGI:1349145).
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present Uniform 3D
Specimen 3E Dh/+: embryonic day 11.5; Dh/Dh+ (more )
Note: Double labeled: purple - Fgf8; blue - Shh (assay MGI:1349145).
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present Regionally restricted 3E In this mutant expression is shifted anteriorly.
Specimen 3F Dh/Dh: embryonic day 11.5; Dh/Dh (more )
Note: Double labeled: purple - Fgf8; blue - Shh (assay MGI:1349145).
Structure Level Pattern Image Note
TS19: footplate Present Regionally restricted 3F In this mutant expression is in a small anterior focus.
Specimen 3G +/+: embryonic day 12.0 (more )
Note: Double labeled: purple - Fgf8; blue - Fgf10 (assay MGI:1349151).
Structure Level Pattern Image Note
TS20: hindlimb Present Regionally restricted 3G Expression is at the apex.
Specimen 3H Dh/+: embryonic day 12.0; Dh/Dh+ (more )
Note: Double labeled: purple - Fgf8; blue - Fgf10 (assay MGI:1349151).
Structure Level Pattern Image Note
TS20: hindlimb Present Regionally restricted 3H In this mutant expression has shifted anteriorly.
Specimen 3I Dh/Dh: embryonic day 12.0; Dh/Dh (more )
Note: Double labeled: purple - Fgf8; blue - Fgf10 (assay MGI:1349151).
Structure Level Pattern Image Note
TS20: hindlimb Present Regionally restricted 3I In this mutant expression is restricted to a small anterior focus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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