Nes<em1(flpo)Awar> - Nervous System Recombinase Activity Detail MGI Mouse

Nes^{em1(flpo)Awar} - Nervous System
Recombinase Activity Detail

Allele Information | Tissue Information | Recombinase Activity | References

Allele Information

Allele:

Nes^{em1(flpo)Awar}
nestin; endonuclease-mediated mutation 1, Rajeshwar Awatramani

Driver: Nes
Type: Endonuclease-mediated (Conditional ready, Recombinase, Reporter)

Synonym:

Nes-LSL-Flpo, PRISM

Molecular description:

Using the CRISPR/Cas9 technology, the Nestin locus in ES cells were targeted to insert loxP, rox, d4GFP, SV40 poly-A, rox, stop cassette, loxP, Flpo, and bGH poly-A at the start codon of the mouse Nes gene. d4GFP is flanked by rox sites and can be removed by crossing to a Dre-deleter mouse. Cre excises the d4GFP and stop cassette, allowing Flpo expression when Nestin is expressed.

Find mice (IMSR):

Mouse Strains: 0 strains available Cell Lines: 0 lines available

Additional Tissues:

Nes^{em1(flpo)Awar} activity also observed in:
head

Tissue Information

Nervous System

Other recombinase alleles with activity in Nervous System tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acsm4^tm1(cre)Hsak, Adcyap1r1^{tm1.1(cre/GFP)Rpa} ...(more)

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

1 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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