Nesem1(flpo)Awar
Endonuclease-mediated Allele Detail
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Symbol: |
Nesem1(flpo)Awar |
Name: |
nestin; endonuclease-mediated mutation 1, Rajeshwar Awatramani |
MGI ID: |
MGI:6740241 |
Synonyms: |
Nes-LSL-Flpo, PRISM |
Gene: |
Nes Location: Chr3:87878400-87887758 bp, + strand Genetic Position: Chr3, 38.78 cM
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Alliance: |
Nesem1(flpo)Awar page
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Germline Transmission: |
Earliest citation of germline transmission:
J:290856
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
Not Specified
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Allele Type: |
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Endonuclease-mediated (Conditional ready, Recombinase, Reporter) |
Mutation: |
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Insertion
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Nesem1(flpo)Awar expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: Using the CRISPR/Cas9 technology, the Nestin locus in ES cells were targeted to insert loxP, rox, d4GFP, SV40 poly-A, rox, stop cassette, loxP, Flpo, and bGH poly-A at the start codon of the mouse Nes gene. d4GFP is flanked by rox sites and can be removed by crossing to a Dre-deleter mouse. Cre excises the d4GFP and stop cassette, allowing Flpo expression when Nestin is expressed.
(J:290856)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Nes
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Nes Mutation: |
81 strains or lines available
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Lentiviral gene trap injected into zygotes.
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Original: |
J:290856 Poulin JF, et al., PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages. Dev Cell. 2020 Jun 22;53(6):740-753.e3 |
All: |
1 reference(s) |
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