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Nphs2tm1.1(icre)Ptb - Renal & Urinary System
Recombinase Activity Detail
Allele Information
Allele:
Nphs2tm1.1(icre)Ptb
nephrosis 2, podocin; targeted mutation 1.1, Paul T Brinkkoetter 		Driver: Nphs2
Type: Targeted (No functional change, Recombinase, Reporter)
Synonym: Nphs2pod.T2A.ciCre.T2A.mTomato
Molecular description: The targeting construct designed to insert a codon improved Cre Recombinase gene (icre) and a membrane-targeted tandem dimer tdTomato (mTomato) under the control of the endogenous Nphs2 promoter using viral T2A-peptides was generated using bacterial artificial chromosome clones from the C57BL/6J RPCIB-731 BAC library. The T2A.icre.T2A.mTomato cassette was inserted between the last codon of exon 8 of Nphs2 and the stop codon. A frt-flanked Puromycin resistance cassette was placed in intron between exon 7 and exon 8. The constitutive knock-in allele was generated after Puromycin resistance cassette was removed by in vivo Flp-mediated removal of the selection marker.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Tissue Information
Renal & Urinary System Other recombinase alleles with activity in Renal & Urinary System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory