Nphs2tm1.1(icre)Ptb
Targeted Allele Detail
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Symbol: |
Nphs2tm1.1(icre)Ptb |
Name: |
nephrosis 2, podocin; targeted mutation 1.1, Paul T Brinkkoetter |
MGI ID: |
MGI:6200320 |
Synonyms: |
Nphs2pod.T2A.ciCre.T2A.mTomato |
Gene: |
Nphs2 Location: Chr1:156138297-156155605 bp, + strand Genetic Position: Chr1, 67.71 cM, cytoband G3
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Alliance: |
Nphs2tm1.1(icre)Ptb page
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Germline Transmission: |
Earliest citation of germline transmission:
J:264681
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
C57BL/6NTac
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Allele Type: |
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Targeted (No functional change, Recombinase, Reporter) |
Mutation: |
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Insertion
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Nphs2tm1.1(icre)Ptb expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: The targeting construct designed to insert a codon improved Cre Recombinase gene (icre) and a membrane-targeted tandem dimer tdTomato (mTomato) under the control of the endogenous Nphs2 promoter using viral T2A-peptides was generated using bacterial artificial chromosome clones from the C57BL/6J RPCIB-731 BAC library. The T2A.icre.T2A.mTomato cassette was inserted between the last codon of exon 8 of Nphs2 and the stop codon. A frt-flanked Puromycin resistance cassette was placed in intron between exon 7 and exon 8. The constitutive knock-in allele was generated after Puromycin resistance cassette was removed by in vivo Flp-mediated removal of the selection marker.
(J:264681)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Nphs2
(mouse)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Nphs2 Mutation: |
30 strains or lines available
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Original: |
J:264681 Koehler S, et al., Construction of a viral T2A-peptide based knock-in mouse model for enhanced Cre recombinase activity and fluorescent labeling of podocytes. Kidney Int. 2017 Jun;91(6):1510-1517 |
All: |
2 reference(s) |
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