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pM-gamma-2-Crl Probe Detail
Nucleotide
Probe/Clone
  • Name
    pM-gamma-2-Crl
  • Sequence Type
    cDNA
  • ID
    MGI:11935
  • Region Covered
    pM[g]>2
  • Child Clone
    gamma-E/F probe
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    614 bp
  • Note
    At low to medium stringency conditions this probe will cross hybridize to Crygf, b, and a.
  • Synonyms
    pMgamma2
Source
  • Species
    mouse, laboratory
  • Strain
    NIH
  • Age
    postnatal day 5-10.0
  • Tissue
    lens
Genes
Cryg crystallin gamma complex
Crygd crystallin, gamma D
Cryge crystallin, gamma E
Crygf crystallin, gamma F
Sequences
K02584 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:10039 Masaki S, et al., Genomics. 1989 Aug;5(2):259-63
Notes: pM[g]2 also detects RFLPs with EcoRI, BamHI, and PstI.
Endonuclease Gene Allele Fragments Strains
DraI Crygd a 4.0kb 129S1/Sv-KitlSl, A/He, AU/SsJ, BALB/cJ, C3H/HeNem, C57BL/6JNga, CAS-QZN, DBA/2, ICR, KK, MOL-M, MOL-Tsm, MOL-Wak, SK/Cam
b 3.4kb 129S1.Cg-CrygeElo KitlSl, CFO
DraI Crygf a 2.4kb 129S1.Cg-CrygeElo KitlSl, 129S1/Sv-KitlSl, A/He, C57BL/6JNga, CAS-QZN, CFO, DBA/2, ICR, KK, MOL-Tsm, MOL-Wak, SK/Cam
b absent AU/SsJ, BALB/cJ, C3H/HeNem, MOL-M
J:10476 Mock B, et al., Genomics. 1990 May;7(1):57-64
Notes: A 330bp PstI portion of the clone was used for Southern blotting.
Endonuclease Gene Allele Fragments Strains
EcoRI Cryg c 5.2kb BALB/c
d 5.0kb DBA/2
References
J:6796 Shinohara T, et al., Multiple gamma-crystallins of the mouse lens: fractionation of mRNAs by cDNA cloning. Proc Natl Acad Sci U S A. 1982 May;79(9):2783-7
J:7698 Breitman ML, et al., Gamma-crystallin family of the mouse lens: structural and evolutionary relationships. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7762-6
J:10039 Masaki S, et al., Linkage analysis of the mutation locus in the eye lens obsolescence (Elo) mouse. Genomics. 1989 Aug;5(2):259-63
J:10476 Mock B, et al., A genetic map of mouse chromosome 1 near the Lsh-Ity-Bcg disease resistance locus. Genomics. 1990 May;7(1):57-64
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory