Experiment
Authors examined the transmission of alternate alleles for selected loci in the EUCIB (BSS) [(C57BL/6J x SPR)F1 x SPR] backcrosse. They then further examined the haplotype data for progeny with recombinant chromosomes. To do so, authors divided mouse Chromosome X into five intervals and for each interval, compared the number of animals carrying the two reciprocal haplotypes that showed a recombination breakpoint in this interval. Only haplotypes with a single recombination breakpoint were analyzed. For the EUCIB (BSS) cross, three successive intervals with equal frequency of reciprocal haplotypes; these intervals, which extend over 40 cM on mouse Chromosome X, were delimited by DXMit91 centromerically and Plp telomerically. Authors propose that at least one DCSX (distortion-controlling locus on the X Chromosome), Dcsx3, is located in the centromeric interval that showed no deviation (DXMit91 - DXMit60), another, Dcsx4, in the telomeric interval (Xist-Plp), and that the middle interval does not have a DCSX. This result provides additional evidence that deviation in the EUCIB (BSS) cross can be expalined by a two loci model and places Dcsx3 within the DXMit91-DXMit60 interval and Dcsx4 within the Xist-Plp1 interval on mouse Chromosome X.
Analysis Tools