Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  1
• Reference
  J:84713 Hitzemann R, et al., Multiple cross mapping (MCM) markedly improves the localization of a QTL for ethanol-induced activation. Genes Brain Behav. 2002 Nov;1(4):214-22
• ID
  MGI:3046646

Genes

Gene	Assay Type
Actre1	reported elsewhere
Actre11	visible phenotype
Actre12	visible phenotype
D1Mit289	PCR amplified length variant
D1Mit425	PCR amplified length variant
D1Mit268	PCR amplified length variant
Kcnj9	reported elsewhere

Notes

• Experiment
  Multiple cross mapping (MCM) was used to identify QTLs associated with ethanol induced activation of locomotor activity. The following F2 crosses were generated and screened for polymorphic markers: (C57BL/6J x DBA/2J)F2, (DBA/2J x LP/J)F2, (BALB/cJ x LP/J)F2, (BALB/cJ x DBA/2J)F2, (C57BL/6J x BALB/cJ)F2, and (C57BL/6J x LP/J)F2.
  
  The data and analysis from the (C57BL/6J x DBA/2J)F2 cross had been previously done and reported in J:71081 and was presented in the article as reference. The QTL identified in that map study was Actre1.
  
  Data from the other 5 crosses was collected in two cohorts, an initial cohort of 400 mice, followed by a second cohort of 200 mice. 250 animals were genotyped from each cross. The threshold for putative QTL was set at p<0.05. Only the (C57BL/6J x BALB/cJ)F2 cross met the threshold. Linkage to ethanol induced locomotor activation was detected on distal mouse Chromosome 1. The QTL information was used to develop an algorithm for sorting and editing the Chr 1 Mit markers. The process yeilded a cluster of markers bewteen 82 and 85 cM. Markers included in this cluster are D1Mit105, 107, 227, 266, 399, 503 and 504.
  
  11.23.2015 Curator Note - When previoulsy identified QTL are cited as confirmed or verified using a different cross than that initially used, we have created a new QTL. We consider each distinct cross to be a separate mapping experiment.
  
  We have named the QTL identified here using the (C57BL/6J x BALB/cJ)F2 population as Actre11. Activity scores were averaged for the two testing trials. The QTL detected in the (C57BL/6J x BALB/cJ)F2 population, Actre11, had a similar profile to that identified in the (C57BL/6J x DBA/2J)F2 cross, Actre1. The BALB/cJ allele appeared to be dominant and was associated with decreased locomotor response.
  
  The authors go on to map the chromosome 1 QTL in an HS sample set previously described in J:71081. (11.23.2015 Curator Note: Since the HS panel is comprised of differing strains than those used to map either Actre1 or Actre11 we also consider the QTL identified in the HS experiment as unique and labeled it Actre12.)
  
  Actre12 mapped to Chromosome 1 peaking between markers D1Mit289 (74.3cM), D1Mit425 (81.6 cM, and D1Mit268 (83.4cM) with a LOD score ranging between 13.5 (D1Mit289-D1Mit425)and 12.7 (D1Mit425-D1Mit268). [Table 1.]
  
  Kcnj9 is a proposed candidate gene.