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| Caption | (A) Schematic illustration of the targeting strategy used to introduce mutation. Diagrams show the wild-type Esr1 locus (WT ERa allele), targeting construct, targeted mutant allele in the ES cells/chimera mice, and F1 mutant allele after ACN cassette self-excision to generate the Esr1tm3.1Ksk allele. The targeting construct contained Esr1 exon 9 (light gray boxes show coding sequence and dark gray box shows 3' UTR), the L543A, L544A mutations ("mutation"), an extra XbaI site (Xb), and a 6xHis-tag epitope (6xHis). The ACN cassette was flanked at the 5' and 3' ends by loxP sites (closed arrowheads). Open box suggests the position of 5' external probe for Southern blot (5'Ex), and pairs of open arrowheads suggest PCR primer sets for 3' external PCR (3'Ex PCR), His-tag PCR, and PCR genotyping. D, DrdI; Xm, XmnI; B, BamHI; Xh, XhoI; H, HindIII. | ||||
| Copyright | This image is from Arao Y, Proc Natl Acad Sci U S A 2011 Sep 6;108(36):14986-91. Copyright 2011 National Academy of Sciences, U.S.A. J:176459 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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