Esr1^tm3.1Ksk
Targeted Allele Detail

Summary

• Symbol: Esr1^tm3.1Ksk
• Name: estrogen receptor 1 (alpha); targeted mutation 3.1, Kenneth S Korach
• MGI ID: MGI:5301452
• Synonyms: AF2ER^KI
• Gene: Esr1 Location: Chr10:4561989-4955633 bp, + strand Genetic Position: Chr10, 2.03 cM
• Alliance: Esr1^tm3.1Ksk page

Uterine abnormalities in Esr1^tm3.1Ksk/Esr1^tm3.1Ksk mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176459
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Missense mutations (leucine to alanine) of the first two codons encoding hydrophobic residues were introduced in exon 9, which encodes the AF-2 motif. Previous in vitro studies showed that the disruption of these residues conferred insensitivity to estradiol. Sequence encoding a carboxy terminal histidine epitope tag was also added to allow for detection of the mutant protein. A self-excising floxed neo cassette that was inserted into the 3' untranslated region was excised via cre recombinase. (J:75132, J:176459)

Generation of the Esr1^tm3.1Ksk allele

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esr1 Mutation: 69 strains or lines available

Notes

ES cells = clone A3 of UG347 ES cells (129S6/SvEvTac)

References

• Original: J:176459 Arao Y, et al., Estrogen receptor {alpha} AF-2 mutation results in antagonist reversal and reveals tissue selective function of estrogen receptor modulators. Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14986-91
• All: 4 reference(s)