Phenotype Image Detail

Image

Caption

Retinal pigment epithelium (RPE) damage, photoreceptor degeneration and amacrine cell alteration in Ccl2^tm1Rol/Ccl2^tm1Rol Cx3cr1^tm1Litt/Cx3cr1^tm1Litt (DKO) mice. (A-F) Confocal images ofRPE/choroidal flatmount from 12-month wild-type (WT) (A), 12-month mutant (B), 18-month mutant (C), and 10 month+light mutant (D, E) and 10-month+light WT (F) mice. Arrows in (E) indicate RPE vacuoles in z-stack images. Scale bar = 50 um. (G) The average number of RPE lesions in different groups of mice. N >/= 6, unpaired Student t test. #, no lesions were detected. (H-J) semithin retinal sections from 18-month WT (H), 18-month mutant (I) and 10-month+light mutant mice (J). ONL, outer nuclear layer; OS, outer segments; Ch, choroid. Asterisks indicate RPE vacuoles. (K, L) Synaptophysin expression in 18-month WT (K) and 18-month mutant (L) mouse retina. OPS, outer plexiform layer; INL, inner nuclear layer. Synaptophysin was observed in ONL in aged mutant mice (arrowheads, L). (M, N) Rhodopsin expression in 18-month WT (M) and mutant (N) mouse retina. Rhodopsin was detected in OPL and ONL layers in aged mutant mouse (N). (O, P) Cone arrestin expression in 18-month WT (O) and mutant (P) mouse retina. (Q, R) Glycine transporter 1 (green) and GABA (red) immunoreactivities in 12-month WT (Q) and mutant (R) mouse retina. A marked reduction in GABA expression was noted in the inner plexiform layer (IPL) of mutant mouse retina. GCL, ganglion cell layer. Scale bar = 25 um.

Copyright

This image is from Chen M, PLoS One 2013;8(4):e61381, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:200877

Associated Alleles

Symbol	Name
Ccl2^tm1Rol	C-C motif chemokine ligand 2; targeted mutation 1, Barrett J Rollins
Cx3cr1^tm1Litt	C-X3-C motif chemokine receptor 1; targeted mutation 1, Dan R Littman

Associated Genotypes

Allelic Composition	Genetic Background
Ccl2^tm1Rol/Ccl2^tm1Rol Cx3cr1^tm1Litt/Cx3cr1^tm1Litt	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6J