Phenotype Image Detail

Image

Caption

Myoblast orientation phenotypes in non-cutaneous maximus (CM)-scapular belt muscles of Fat1^{Gt(KST249)Byg}/Fat1^{Gt(KST249)Byg} Tg(Myl1-lacZ)1Ibdml/0 (Fat1^LacZ/LacZ) embryos. Whole mount in situ hybridization with a MyoD RNA probe on wild-type (WT) (A, C, E) or mutant (B, D, F) E12.5 embryo. (A, B) Low power magnification micrographs showing a side view of shoulder area. Anterior is to the left, dorsal is to the top. (C, D) Higher power magnification micrographs showing an enlargement of the corresponding boxed areas in (A and B), respectively. Mutant embryos present numerous dispersed myoblasts in ectopic positions in the shoulder area, either as individual cells (red arrows), or clustered and forming ectopic muscles(orange arrows). (E,F) Higher (X63) magnification views of the corresponding boxed areas within the trapezius muscles in (C and D) showing misoriented myoblasts in mutant embryos. Scale bars: (A-B) 0.5 um; (C, D) 50 um; (E, F) 10 um.

Copyright

This image is from Caruso N, PLoS Genet 2013 Jun;9(6):e1003550, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:199157

Associated Alleles

Symbol	Name
Fat1^Gt(KST249)Byg	FAT atypical cadherin 1; gene trap KST249, BayGenomics
Tg(Myl1-lacZ)1Ibdml	transgene insertion 1, Developmental Biology Institue of Marseilles-Luminy

Associated Genotypes

Allelic Composition	Genetic Background
Fat1^Gt(KST249)Byg/Fat1^Gt(KST249)Byg Tg(Myl1-lacZ)1Ibdml/0	involves: 129P2/OlaHsd