Phenotype Image Detail

Image

Caption

Compared with control Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt (Ns^f/f) embryos, Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt Tg(Nes-cre)1Kln/0 (neNS^cko) embryos begin to show a decreased cellularity in the telencephalic (T), diencephalic (D), mesencephalic (M), and rhombencephalic (R) neuroepithelium at E12.5. Squares indicate regions shown in G. At E14.5 (D) and E16.5 (E), mutant (neNS^cko) embryos exhibit severe brain defects throughout the neural axis. Ce, cerebellar primordium. (F) At E12.5, the forebrains of mutant embryos contain much less sphere-forming neural stem cells (NSCs) than that of control (NS^f/f) embryos. (G) A notable phenotype is the increase of gamma-H2AX+ cells in the ventricular zone of the E12.5 neuroepithelium. The region shown here is close to the midbrain roof. (Scale bars: 500 um in C, 1 mm in D and E, and 100 um in F and G.) Bar graphs, mean +/- SEM; *P < 0.01; **P < 0.001; ***P < 0.0001.

Copyright

This image is from Meng L, Proc Natl Acad Sci U S A 2013 Jul 9;110(28):11415-20. Copyright 2013 National Academy of Sciences, U.S.A. J:198698

Associated Alleles

Symbol	Name
Gnl3^tm2.1Rylt	guanine nucleotide binding protein nucleolar 3; targeted mutation 2.1, Robert Y L Tsai
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Gnl3^tm2.1Rylt/Gnl3^tm2.1Rylt Tg(Nes-cre)1Kln/0	involves: 129 * C57BL/6 * CBA