Phenotype Image Detail

Image

Caption

(E and F) Immunostaining for pAkt in control (WT) mice 24h after hypoxia-ischemia (HI; nonischemic and ischemic cortex) shows increased numbers of pAkt-positive neurons in the periinfarct region. (G and H) In Ndfip1^tm1Sest/Ndfip1^tm1Sest Tg(Nes-cre)1Kln/0 (KO) mice, fewer pAkt-positive neurons are observed in the periinfarct region after HI. (E-H) Insets show magnifications of the boxed areas. Bar, 30 um. (I) Quantification of pAkt levels in the ischemic cortex 24h after HI shows that fewer neurons activate Akt in the periinfarct region in mutant mice. **, P < 0.005 compared with controls (two-tailed unpaired Student's t test; n = 5 per genotype). Values are mean +/- SEM throughout.

Copyright

This image is from Howitt J, J Cell Biol 2012 Jan 9;196(1):29-36, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:180657

Associated Alleles

Symbol	Name
Ndfip1^tm1Sest	Nedd4 family interacting protein 1; targeted mutation 1, Seong-Seng Tan
Tg(Nes-cre)1Kln	transgene insertion 1, Rudiger Klein

Associated Genotypes

Allelic Composition	Genetic Background
Ndfip1^tm1Sest/Ndfip1^tm1Sest Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL