Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of En2. (This text reflects annotations as of Wednesday, January 23, 2013.)

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of En2
  • participates in the following biological processes:
    • positive regulation of transcription from RNA polymerase II promoter ^[2]
• Researchers have inferred, based on genetic interactions, that the gene product of En2
  • participates in the following biological processes:
    • hindbrain development based on interactions with En1 ^[5]
    • midbrain development based on interactions with En1 ^{[1, 5]}
    • neuron development based on interactions with En1 ^[3]
    • neuron differentiation based on interactions with En1 ^{[1, 4, 5]}

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of En2:
  • is located in the following cellular components:
    • membrane
    • nucleus

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Summary text based on GO annotations supported by structural data

• En2 encodes a protein or proteins that contain the following InterPro domains: Homeobox engrailed, C-terminal, Homeobox engrailed-type, conserved site, Homeobox, conserved site, Homeodomain, Homeodomain engrailed, Homeodomain, metazoa, Homeodomain-like, indicating that the gene product:
  • participates in the following biological processes:
    • multicellular organismal development
    • regulation of transcription, DNA-dependent
  • performs the following molecular functions:
    • DNA binding
    • sequence-specific DNA binding
    • sequence-specific DNA binding transcription factor activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe En2 indicates that it:
  • participates in the following biological processes:
    • multicellular organismal development
  • performs the following molecular functions:
    • DNA binding

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References

1. Alberi L et al. (2004) Engrailed genes are cell-autonomously required to prevent apoptosis in mesencephalic dopaminergic neurons. Development, 131:3229-36. (PubMed:15175251)
2. Kury P et al. (1997) Protease nexin-1 is expressed at the mouse met-/mesencephalic junction and FGF signaling regulates its promoter activity in primary met-/mesencephalic cells. Development, 124:1251-62. (PubMed:9102311)
3. Simon HH et al. (2001) Fate of midbrain dopaminergic neurons controlled by the engrailed genes. J Neurosci, 21:3126-34. (PubMed:11312297)
4. Simon HH et al. (2004) Midbrain dopaminergic neurons: control of their cell fate by the engrailed transcription factors. Cell Tissue Res, 318:53-61. (PubMed:15340832)
5. Simon HH et al. (2005) Engrailed genes control developmental fate of serotonergic and noradrenergic neurons in mid- and hindbrain in a gene dose-dependent manner. Mol Cell Neurosci, 28:96-105. (PubMed:15607945)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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