Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Tbx20. (This text reflects annotations as of Wednesday, August 14, 2013.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of July 13, 2011. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Tbx20
  • participates in the following biological processes:
    • negative regulation of SMAD protein complex assembly ^[5]
    • negative regulation of transcription, DNA-dependent ^{[1, 3]}
    • positive regulation of transcription, DNA-dependent ^{[1, 9]}
  • performs the following molecular functions:
    • DNA binding ^{[1, 3]}
    • RNA polymerase II regulatory region sequence-specific DNA binding ^[8]
    • RNA polymerase II transcription coactivator activity ^[8]
    • sequence-specific DNA binding transcription factor activity ^{[1, 3]}
  • is located in the following cellular components:
    • cytoplasm ^[8]
    • nucleus ^[8]
• Researchers have inferred, based on physical interactions, that the gene product of Tbx20
  • performs the following molecular functions:
    • RNA polymerase II activating transcription factor binding ^{[2, 8]}
    • RNA polymerase II transcription factor binding ^{[2, 8]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Tbx20
  • participates in the following biological processes:
    • aortic valve development ^[9]
    • blood circulation ^[7]
    • cardiac chamber formation ^{[4, 5]}
    • cardiac muscle tissue morphogenesis ^[9]
    • cardiac right ventricle morphogenesis ^[9]
    • cardiac septum development ^[9]
    • cell proliferation ^[1]
    • dorsal/ventral pattern formation ^[4]
    • embryonic heart tube development ^[7]
    • embryonic heart tube elongation ^[4]
    • embryonic heart tube morphogenesis ^[4]
    • endocardial cushion formation ^[9]
    • endocardial cushion morphogenesis ^[4]
    • heart looping ^{[1, 4, 9]}
    • muscle contraction ^[7]
    • negative regulation of transcription from RNA polymerase II promoter ^[4]
    • negative regulation of transcription, DNA-dependent ^[9]
    • neuron migration ^[6]
    • outflow tract morphogenesis ^[9]
    • outflow tract septum morphogenesis ^[9]
    • patterning of blood vessels ^[7]
    • pericardium morphogenesis ^[4]
    • positive regulation of cardiac muscle cell proliferation ^[4]
    • pulmonary valve formation ^[9]
    • tricuspid valve development ^[9]
    • visceral motor neuron differentiation ^[9]
• Researchers have inferred, based on genetic interactions, that the gene product of Tbx20
  • participates in the following biological processes:
    • embryonic heart tube development based on interactions with Nkx2-5 ^[7]
    • negative regulation of transcription from RNA polymerase II promoter based on interactions with Smad4 ^[5]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Tbx20:
  • participates in the following biological processes:
    • aortic valve morphogenesis
    • atrial septum morphogenesis
    • foramen ovale closure
    • outflow tract septum morphogenesis
    • pulmonary vein morphogenesis
  • performs the following molecular functions:
    • RNA polymerase II regulatory region sequence-specific DNA binding
    • RNA polymerase II transcription coactivator activity

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Tbx20 indicates that it:
  • participates in the following biological processes:
    • multicellular organismal development
    • transcription, DNA-dependent

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References

1. Cai CL et al. (2005) T-box genes coordinate regional rates of proliferation and regional specification during cardiogenesis. Development, 132:2475-87. (PubMed:15843407)
2. Pereira LA et al. (2011) Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity. PLoS One, 6:e28394. (PubMed:22164283)
3. Plageman TF Jr et al. (2004) Differential expression and function of Tbx5 and Tbx20 in cardiac development. J Biol Chem, 279:19026-34. (PubMed:14978031)
4. Singh MK et al. (2005) Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2. Development, 132:2697-707. (PubMed:15901664)
5. Singh R et al. (2009) Tbx20 interacts with smads to confine tbx2 expression to the atrioventricular canal. Circ Res, 105:442-52. (PubMed:19661464)
6. Song MR et al. (2006) T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. Development, 133:4945-55. (PubMed:17119020)
7. Stennard FA et al. (2005) Murine T-box transcription factor Tbx20 acts as a repressor during heart development, and is essential for adult heart integrity, function and adaptation. Development, 132:2451-62. (PubMed:15843414)
8. Stennard FA et al. (2003) Cardiac T-box factor Tbx20 directly interacts with Nkx2-5, GATA4, and GATA5 in regulation of gene expression in the developing heart. Dev Biol, 262:206-24. (PubMed:14550786)
9. Takeuchi JK et al. (2005) Tbx20 dose-dependently regulates transcription factor networks required for mouse heart and motoneuron development. Development, 132:2463-74. (PubMed:15843409)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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