Aldh1b1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:192460 Ioannou M, et al., ALDH1B1 is a potential stem/progenitor marker for multiple pancreas progenitor pools. Dev Biol. 2013 Feb 1;374(1):153-63
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6405178
Gene symbol:	Aldh1b1
Gene name:	aldehyde dehydrogenase 1 family, member B1

Antibody:	anti-Aldh1b1
Detection system:	Alexa Fluor 488

Results

S2A ©

Specimen S2A: embryonic day 14.5 (more

)
Note: Triple labeled: green - ALDH1B1 protein; red - Aldh1b1 RNA (assay MGI:6405174); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present	Regionally restricted	S2A	All expressing cells also express Aldh1b1 RNA.

S2B ©

Specimen S2B: embryonic day 14.5 (more

)
Note: Triple labeled: green - ALDH1B1 protein; red - Aldh1b1 RNA (assay MGI:6405174); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present	Regionally restricted	S2B	All expressing cells also express Aldh1b1 RNA.

1C ©

Specimen 1C: embryonic day 9.5 (more

)
Note: Double labeled: green - Aldh1b1; red - Pdx1 (assay MGI:6405176).

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium dorsal bud	Present	Regionally restricted	1C	Authors report co-expression with Pdx1.

1E ©

Specimen 1E: embryonic day 9.5; Pdx1^tm1Macd/Pdx1^tm1Macd (more

)
Note: Double labeled: green - Aldh1b1; red - Pdx1 (assay MGI:6405176).

Structure	Level	Pattern	Image	Note
TS15: pancreas primordium dorsal bud	Absent		1E

1G ©

Specimen 1G: embryonic day 10.5 (more

)
Note: Double labeled: green - Aldh1b1; red - Pdx1 (assay MGI:6405176).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium dorsal bud	Present	Regionally restricted	1G	Authors report continued co-expression with Pdx1.
TS17: pancreas primordium ventral bud	Present	Regionally restricted	1G	Authors report continued co-expression with Pdx1.

1I ©

Specimen 1I: embryonic day 10.5; Pdx1^tm1Macd/Pdx1^tm1Macd (more

)
Note: Double labeled: green - Aldh1b1; blue - Isl1 (assay MGI:6405198).

Structure	Level	Pattern	Image	Note
TS17: pancreas primordium dorsal bud	Absent		1I
TS17: pancreas primordium ventral bud	Absent		1I

2A ©

2B ©

Specimen 2A/B: embryonic day 12.5 (more

)
Note: Double labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191).

Structure	Level	Pattern	Image	Note
TS20: pancreas epithelium	Present	Regionally restricted	2A , 2B	Expression exclusively in epithelium in both tips and trunks of developing pancreas, stronger at tips. No expression in mesenchyme.

2C ©

2D ©

Specimen 2C/D: embryonic day 14.5 (more

)
Note: Double labeled: green - Aldh1b1; red - Nkx6-1 (assay MGI:6405217).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted	2C , 2D	Expression exclusively in epithelium in both tips and trunks of developing pancreas, stronger at tips. Expression in most but not all trunk cells. No expression in mesenchyme.

2E ©

Specimen 2E: embryonic day 14.5 (more

)
Note: Double labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted	2E	Expression in trunk cells.

2F ©

2G ©

Specimen 2F/G: embryonic day 14.5 (more

)
Note: Double labeled: green - Aldh1b1; red - Nkx6-1 (assay MGI:6405217).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted	2F , 2G

2H ©

Specimen 2H: embryonic day 14.5; Neurog3^tm1Fgu/Neurog3^tm1Fgu (more

)
Note: Double labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted	2H	No expression in trunk cells.

2I ©

2J ©

Specimen 2I/J: embryonic day 14.5; Neurog3^tm1Fgu/Neurog3^tm1Fgu (more

)
Note: Triple labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191); blue - Neurog3 (assay MGI:6405220).

Structure	Level	Pattern	Image	Note
TS22: pancreas epithelium	Present	Regionally restricted	2J , 2I	No expression in trunk cells.

Specimen E16.5 not shown: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: pancreas epithelium	Present	Regionally restricted		Expression restricted at tips.

Specimen E18.5 not shown: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: pancreas	Absent			Authors report expression was completely abolished by the end of the gestation period.

6A ©

Specimen 6A: postnatal adult (more

)
Note: Triple labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191); blue - DAPI

Structure	Level	Pattern	Image	Note
TS28: pancreas	Ambiguous		6A

6B ©

Specimen 6B: postnatal adult (more

)
Note: Triple labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191); blue - DAPI

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Single cells	6B	Expression persisted in rare elongated cells in adult pancreas.

6C ©

Specimen 6C: postnatal adult (more

)
Note: Triple labeled: green - Aldh1b1; red - PNA; blue - DAPI

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Single cells	6C	Expression in rare cells that were positive for PNA.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23