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Gene Expression Data
Assay Details
Assay
Reference: J:192460 Ioannou M, et al., ALDH1B1 is a potential stem/progenitor marker for multiple pancreas progenitor pools. Dev Biol. 2013 Feb 1;374(1):153-63
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6405178
Gene symbol: Aldh1b1
Gene name: aldehyde dehydrogenase 1 family, member B1
Antibody: anti-Aldh1b1
Detection system: Alexa Fluor 488
Results
Specimen S2A: embryonic day 14.5 (more )
Note: Triple labeled: green - ALDH1B1 protein; red - Aldh1b1 RNA (assay MGI:6405174); blue - DAPI.
Structure Level Pattern Image Note
TS22: pancreas Present Regionally restricted S2A All expressing cells also express Aldh1b1 RNA.

Specimen S2B: embryonic day 14.5 (more )
Note: Triple labeled: green - ALDH1B1 protein; red - Aldh1b1 RNA (assay MGI:6405174); blue - DAPI.
Structure Level Pattern Image Note
TS22: pancreas Present Regionally restricted S2B All expressing cells also express Aldh1b1 RNA.

Specimen 1C: embryonic day 9.5 (more )
Note: Double labeled: green - Aldh1b1; red - Pdx1 (assay MGI:6405176).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Present Regionally restricted 1C Authors report co-expression with Pdx1.

Specimen 1E: embryonic day 9.5; Pdx1tm1Macd/Pdx1tm1Macd (more )
Note: Double labeled: green - Aldh1b1; red - Pdx1 (assay MGI:6405176).
Structure Level Pattern Image Note
TS15: pancreas primordium dorsal bud Absent 1E

Specimen 1G: embryonic day 10.5 (more )
Note: Double labeled: green - Aldh1b1; red - Pdx1 (assay MGI:6405176).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Present Regionally restricted 1G Authors report continued co-expression with Pdx1.
TS17: pancreas primordium ventral bud Present Regionally restricted 1G Authors report continued co-expression with Pdx1.

Specimen 1I: embryonic day 10.5; Pdx1tm1Macd/Pdx1tm1Macd (more )
Note: Double labeled: green - Aldh1b1; blue - Isl1 (assay MGI:6405198).
Structure Level Pattern Image Note
TS17: pancreas primordium dorsal bud Absent 1I
TS17: pancreas primordium ventral bud Absent 1I

Specimen 2A/B: embryonic day 12.5 (more )
Note: Double labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191).
Structure Level Pattern Image Note
TS20: pancreas epithelium Present Regionally restricted 2A , 2B Expression exclusively in epithelium in both tips and trunks of developing pancreas, stronger at tips. No expression in mesenchyme.

Specimen 2C/D: embryonic day 14.5 (more )
Note: Double labeled: green - Aldh1b1; red - Nkx6-1 (assay MGI:6405217).
Structure Level Pattern Image Note
TS22: pancreas epithelium Present Regionally restricted 2C , 2D Expression exclusively in epithelium in both tips and trunks of developing pancreas, stronger at tips. Expression in most but not all trunk cells. No expression in mesenchyme.

Specimen 2E: embryonic day 14.5 (more )
Note: Double labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191).
Structure Level Pattern Image Note
TS22: pancreas epithelium Present Regionally restricted 2E Expression in trunk cells.

Specimen 2F/G: embryonic day 14.5 (more )
Note: Double labeled: green - Aldh1b1; red - Nkx6-1 (assay MGI:6405217).
Structure Level Pattern Image Note
TS22: pancreas epithelium Present Regionally restricted 2F , 2G

Specimen 2H: embryonic day 14.5; Neurog3tm1Fgu/Neurog3tm1Fgu (more )
Note: Double labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191).
Structure Level Pattern Image Note
TS22: pancreas epithelium Present Regionally restricted 2H No expression in trunk cells.

Specimen 2I/J: embryonic day 14.5; Neurog3tm1Fgu/Neurog3tm1Fgu (more )
Note: Triple labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191); blue - Neurog3 (assay MGI:6405220).
Structure Level Pattern Image Note
TS22: pancreas epithelium Present Regionally restricted 2J , 2I No expression in trunk cells.

Specimen E16.5 not shown: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: pancreas epithelium Present Regionally restricted Expression restricted at tips.

Specimen E18.5 not shown: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: pancreas Absent Authors report expression was completely abolished by the end of the gestation period.

Specimen 6A: postnatal adult (more )
Note: Triple labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191); blue - DAPI
Structure Level Pattern Image Note
TS28: pancreas Ambiguous 6A

Specimen 6B: postnatal adult (more )
Note: Triple labeled: green - Aldh1b1; red - Cdh1 (assay MGI:6405191); blue - DAPI
Structure Level Pattern Image Note
TS28: pancreas Present Single cells 6B Expression persisted in rare elongated cells in adult pancreas.

Specimen 6C: postnatal adult (more )
Note: Triple labeled: green - Aldh1b1; red - PNA; blue - DAPI
Structure Level Pattern Image Note
TS28: pancreas Present Single cells 6C Expression in rare cells that were positive for PNA.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/19/2021
MGI 6.17
The Jackson Laboratory