Probe:	unc5d cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: auditory cortex	Strong		1A
TS28: primary somatosensory cortex	Strong		1A
TS28: visual cortex	Strong		1A

Structure	Level	Pattern	Image	Note
TS28: parietal cortex	Strong		2A
TS28: neocortex	Weak	Widespread	2A	Weak expression in all cortical areas.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Strong		2C
TS28: primary somatosensory cortex	Strong		2C

Structure	Level	Pattern	Image	Note
TS28: occipital cortex	Strong		2E
TS28: neocortex	Weak	Widespread	2E	Weak expression in all cortical areas.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Strong		2G
TS28: visual cortex	Strong		2G
TS28: cortical layer II	Present		2G	Weaker expression.
TS28: cortical layer III	Present		2G	Weaker expression.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present	Uniform	3A	Uniformly distributed along the anterior-posterior axis.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex subventricular zone	Weak		3B	Weakest expression.
TS27: brain white matter	Weak		3B	Weakest expression. Expressed in a part of the white matter.
TS27: neocortex	Weak	Regionally restricted	3B	Expressed in the upper part.
TS27: frontal cortex	Weak		3B

Structure	Level	Pattern	Image	Note
TS27: occipital cortex	Present	Non-Uniform	3C	Discontinuous expression pattern.
TS27: parietal cortex	Present	Non-Uniform	3C	Discontinuous expression pattern.
TS27: frontal cortex	Weak		3C

Structure	Level	Pattern	Image	Note
TS28: frontal cortex	Weak		3D
TS28: occipital cortex	Present	Non-Uniform	3D	Discontinuous expression pattern. More intense signals.
TS28: parietal cortex	Present	Non-Uniform	3D	Discontinuous expression pattern. More intense signals.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Regionally restricted	4A , 4C	Expressed in thalamocortical axons. Expression largely, but not completely, overlapped with Slc6a4 immunopositive areas.

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Strong	Regionally restricted	4F	Intense, but not homogeneous, signals are observed in the barrel walls and partially in the hollows. The signals are hardly seen in the septal regions.

Structure	Level	Pattern	Image	Note
TS28: parietal cortex	Present	Regionally restricted	6C	Expressed preferentially in the sensory cortices. Partially overlaps with that of Ntn4.

Structure	Level	Pattern	Image	Note
TS28: occipital cortex	Present	Regionally restricted	6G	Expressed preferentially in the sensory cortices. Almost completely overlaps with that of Ntn4.
TS28: ventral lateral geniculate nucleus	Present		6G

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Present		6I right
TS28: primary somatosensory cortex	Present		6I right
TS28: cortical layer II	Weak		6I right
TS28: cortical layer III	Weak		6I right

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Present		6J right
TS28: visual cortex	Present		6J right
TS28: cortical layer II	Weak		6J right
TS28: cortical layer III	Weak		6J right

Structure	Level	Pattern	Image	Note
TS28: auditory cortex	Present			Relatively weaker expression compard with that of the primary somatosensory and visual areas.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex subventricular zone	Weak
TS27: frontal cortex	Weak
TS27: brain white matter	Weak	Regionally restricted		Expressed in a part of the white matter.