About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:130430 Andrews W, et al., The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons. Dev Biol. 2008 Jan 15;313(2):648-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6188844
Gene symbol: Robo1
Gene name: roundabout guidance receptor 1
Antibody: Robo1 antibody
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Rabbit anti-Robo1 MGI:6188838 may have been used in this assay.
Results
Specimen 1 Robo1 E13.5 Rostral: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebral cortex Present Widespread 1_Robo1 E13.5 Rostral Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen 1 Robo1 E13.5 Middle: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: medial ganglionic eminence Strong Regionally restricted 1_Robo1 E13.5 Middle Strong expression throughout mantle zone of medial ganglionic eminence.

Specimen 1 Robo1 E13.5 Caudal: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: telencephalon Present Widespread 1_Robo1 E13.5 Caudal Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen 1 Robo1 E15.5 Rostral: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: basal ganglia Present Widespread 1_Robo1 E15.5 Rostral Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex Present Widespread 1_Robo1 E15.5 Rostral Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen 1 Robo1 E15.5 Middle: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: basal ganglia Present Widespread 1_Robo1 E15.5 Middle Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex Present Widespread 1_Robo1 E15.5 Middle Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen 1 Robo1 E15.5 Caudal: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: basal ganglia Present Widespread 1_Robo1 E15.5 Caudal Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS23: cerebral cortex Present Widespread 1_Robo1 E15.5 Caudal Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen Robo1 E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex Strong Regionally restricted Strong expression in preplate layer.
TS23: cerebral cortex mantle layer Strong
TS23: cortical subplate Strong
TS23: cerebral cortex intermediate zone Strong Regionally restricted Strong expression in tangential migratory routes of interneurons, especially within intermediate zone/subventricular zone.
TS23: cerebral cortex subventricular zone Strong Regionally restricted Strong expression in tangential migratory routes of interneurons, especially within intermediate zone/subventricular zone.

Specimen 1 Robo1 E17.5 Rostral: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: basal ganglia Present Widespread 1_Robo1 E17.5 Rostral Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex Present Widespread 1_Robo1 E17.5 Rostral Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen 1 Robo1 E17.5 Middle: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: basal ganglia Present Widespread 1_Robo1 E17.5 Middle Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex Present Widespread 1_Robo1 E17.5 Middle Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen 1 Robo1 E17.5 Caudal: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: basal ganglia Present Widespread 1_Robo1 E17.5 Caudal Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.
TS25: cerebral cortex Present Widespread 1_Robo1 E17.5 Caudal Authors report expression throughout the rostral-caudal extend of differentiating basal ganglia and cortex.

Specimen Robo1 E17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: telencephalon Present Regionally restricted Expression somewhat downregulated in ventral telencephalon.
TS25: cerebral cortex subventricular zone Strong

Specimen 2A: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cortical plate Present 2A Expression in interneurons throughout the cortical anlage.
TS23: cerebral cortex intermediate zone Present Regionally restricted 2A Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.
TS23: cerebral cortex subventricular zone Present Regionally restricted 2A Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.
TS23: cortical subplate Present Regionally restricted 2A Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.

Specimen 2B: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cortical plate Present Regionally restricted 2B Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.
TS23: cortical subplate Present Regionally restricted 2B Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.

Specimen 2C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Present Regionally restricted 2C Expression in cells appearing to be leaving the subplate and intermediate zone/subventricular zone migratory stream, moving into developing cortical plate and ventricular zone.

Specimen S1J +/+: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brain Present S1J +/+

Specimen S1J -/-: embryonic day 12.5; Robo1tm1Wian/Robo1tm1Wian (more )
Structure Level Pattern Image Note
TS20: brain Absent S1J -/-


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
07/29/2020
MGI 6.15
The Jackson Laboratory