Probe:	Prickle1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	2A	Expression is in the posterior primitive streak.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from head mesoderm	Present		2B
TS14: pronephros	Present		2B
TS14: future spinal cord	Present	Regionally restricted	2B	Expression is in the posterior neural tube.
TS14: embryo mesenchyme	Present	Regionally restricted	2B	Expression is in the mesoderm posterior to the node.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary component mesenchyme	Strong		2C
TS17: neural tube	Strong		2C
TS17: tail	Strong		2C
TS17: somite	Strong		2C	Expression is in the newly formed epithelial somites.
TS17: apical ectodermal ridge	Present		2C
TS17: embryo mesenchyme	Present	Regionally restricted	2C	Expression is in the distal mesenchyme.
TS17: limb mesenchyme	Present	Regionally restricted	2C	Expression is in the most distal mesenchyme around the growing buds.

Structure	Level	Pattern	Image	Note
TS17: myotome	Present		3A

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present		3C

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted	3E	Expression is in the mesenchymal cells of the most distal tip of the limb buds.
TS20: hindlimb pre-cartilage condensation	Present	Regionally restricted	3E	Expression is in chondrocytes.

Structure	Level	Pattern	Image	Note
TS23: digit mesenchyme	Present	Regionally restricted	3G	Expression is in the mesenchymal cells surrounding the distal tip of each bone primordia and in the region of future joints.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present		3H
TS20: vertebral cartilage condensation	Present		3H

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	4A	Expression is in differentiating neurons located in the basal plate of the neural tube.
TS17: otocyst	Present	Regionally restricted	4A	Expression is in the forming endolymphatic duct and sac of the inner ear.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch maxillary component mesenchyme	Strong		4B
TS17: neural tube	Present	Regionally restricted	4B	Expression is in differentiating neurons.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	4C	Expression is in nuclei-like spots at the boundary between mesencephalic vesicle and prosencephalon.
TS20: mesencephalic vesicle	Present	Regionally restricted	4C	Expression is in nuclei-like spots at the boundary between mesencephalic vesicle and prosencephalon.

Structure	Level	Pattern	Image	Note
TS20: endolymphatic appendage	Present		4D
TS20: endolymphatic sac	Present		4D
TS20: pons	Present		4D

Structure	Level	Pattern	Image	Note
TS20: pituitary gland	Present		4E

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted	4F	Expression is in the ocular premuscle mass.
TS20: cornea	Present	Regionally restricted	4F	Expression is in the mesothelial layer of the cornea and lens.
TS20: lens vesicle	Present	Regionally restricted	4F	Expression is in the mesothelial layer of the cornea and lens.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present	Regionally restricted		Expression is in facial cartilage primordia.
TS20: lung	Present
TS20: metanephros	Present
TS20: nephric duct	Present
TS20: urogenital ridge	Present