Probe:	Prickle2 cDNA1
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: nervous system	Moderate	Diffuse	1I
TS17: limb	Present		1I
TS17: embryo	Present	Non-Uniform	1I	Expression was detected at moderate intensity in neural tissue, and to a lesser level in non-neural tissue, particularly in limb buds.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Moderate	Diffuse	2X	Expression was moderately and diffusely expressed in neural tissue, more at caudal than rostral levels, and more in migration and postmitotic differentiation fields than in ventricular zones.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Moderate	Diffuse	2Y	Expression was moderately and diffusely expressed in neural tissue, more at caudal than rostral levels, and more in migration and postmitotic differentiation fields than in ventricular zones.

Structure	Level	Pattern	Image	Note
TS22: organ system	Trace

Structure	Level	Pattern	Image	Note
TS25: brainstem	Present	Regionally restricted		Expression was present in the cochleovestibular nuclei and very low or undetectable in the reticular formation.
TS25: cerebellum	Present	Regionally restricted		Expression was present in the external granule layer.
TS25: diencephalon ventricular layer	Ambiguous			Expression was very low or undetectable.
TS25: midbrain tegmentum	Ambiguous			Expression was very low or undetectable.
TS25: midbrain ventricular layer	Ambiguous			Expression was very low or undetectable.
TS25: tectum	Ambiguous			Expression was very low or undetectable.
TS25: thalamus	Present	Regionally restricted		Expression was detected in the dorsal and ventral thalamus.
TS25: hypothalamus	Present
TS25: retina	Ambiguous	Regionally restricted		Expression was very low or undetectable in the ventricular zone and ganglion cells.
TS25: cortical plate	Present	Regionally restricted		Expression was detected in the cortical plate ventricular zone/subventricular zone and in the cortical plate proper.
TS25: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum ventricular zone/subventricular zone and in the striatum.
TS25: main olfactory bulb	Ambiguous	Regionally restricted		Expression was very low or undetectable in the olfactory bulb and the mitral cells.

Structure	Level	Pattern	Image	Note
TS25: brain	Weak	Widespread	4G3
TS25: brainstem	Strong	Regionally restricted	4G3	Expression was strong in the cochleovestibular nuclei.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong	Regionally restricted	4G4	Expression was strong in the dorsal thalamic nuclei.
TS25: cortical plate	Strong	Widespread	4G4	Expression was strong in all sectors of the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Graded	5M	Expression in the cortical plate was high with a rostral to caudal decreasing gradient.
TS27: brain	Weak	Regionally restricted	5M	Expression was weak in areas of the brain, including all periventricular zones and the rostral migratory stream.
TS27: rostral migratory stream	Weak		5M
TS27: cerebellum external granule cell layer	Weak		5M

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present
TS28: dentate gyrus	Present
TS28: hippocampus pyramidal cell layer	Present
TS28: main olfactory bulb granule cell layer	Present
TS28: main olfactory bulb mitral cell layer	Present
TS28: diencephalon	Ambiguous	Regionally restricted		Expression was very low or undetectable in the diencephalon ventricular zone.
TS28: dorsal thalamus	Ambiguous			Expression was very low or undetectable.
TS28: hypothalamus	Ambiguous			Expression was very low or undetectable.
TS28: prethalamus	Ambiguous			Expression was very low or undetectable.
TS28: cerebral cortex	Present	Regionally restricted		Expression was detected in the cortical plate proper but was very low or undetectable in the cortical plate ventricular zone/subventricular zone.
TS28: corpus striatum	Ambiguous	Regionally restricted		Expression was very low or undetectable in the striatum and the ventricular zone/subventricular zone.
TS28: rostral migratory stream	Ambiguous	Regionally restricted		Expression was very low or undetectable in the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Weak	Regionally restricted	6V	Expression was weak in the outer half of the neocortical ribbon at the lateral aspect of the hemisphere.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Weak	Regionally restricted	6W	Expression was weak in the outer half of the neocortical ribbon at the lateral aspect of the hemisphere.

Structure	Level	Pattern	Image	Note
TS28: brain	Weak		6X

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	7H	Expression was prominent in the most superficial layers but undetectable in deep layers.