Gene Expression Data
Assay Details
Assay
|
Reference:
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J:153498
Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
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Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:4825634
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Gene symbol:
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Itch
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Gene name:
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itchy, E3 ubiquitin protein ligase
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Probe:
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T30618
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.
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|
Results
|
Specimen
euxassay_009772_01:
embryonic day 14.5
(more )
Specimen
euxassay_009772_01:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_02:
embryonic day 14.5
(more )
Specimen
euxassay_009772_02:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_03:
embryonic day 14.5
(more )
Specimen
euxassay_009772_03:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_04:
embryonic day 14.5
(more )
Specimen
euxassay_009772_04:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_05:
embryonic day 14.5
(more )
Specimen
euxassay_009772_05:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_06:
embryonic day 14.5
(more )
Specimen
euxassay_009772_06:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_07:
embryonic day 14.5
(more )
Specimen
euxassay_009772_07:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_08:
embryonic day 14.5
(more )
Specimen
euxassay_009772_08:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_09:
embryonic day 14.5
(more )
Specimen
euxassay_009772_09:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_10:
embryonic day 14.5
(more )
Specimen
euxassay_009772_10:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_11:
embryonic day 14.5
(more )
Specimen
euxassay_009772_11:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_12:
embryonic day 14.5
(more )
Specimen
euxassay_009772_12:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_13:
embryonic day 14.5
(more )
Specimen
euxassay_009772_13:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_14:
embryonic day 14.5
(more )
Specimen
euxassay_009772_14:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_15:
embryonic day 14.5
(more )
Specimen
euxassay_009772_15:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_16:
embryonic day 14.5
(more )
Specimen
euxassay_009772_16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_17:
embryonic day 14.5
(more )
Specimen
euxassay_009772_17:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_18:
embryonic day 14.5
(more )
Specimen
euxassay_009772_18:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_19:
embryonic day 14.5
(more )
Specimen
euxassay_009772_19:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_20:
embryonic day 14.5
(more )
Specimen
euxassay_009772_20:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_21:
embryonic day 14.5
(more )
Specimen
euxassay_009772_21:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_22:
embryonic day 14.5
(more )
Specimen
euxassay_009772_22:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_23:
embryonic day 14.5
(more )
Specimen
euxassay_009772_23:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
euxassay_009772_24:
embryonic day 14.5
(more )
Specimen
euxassay_009772_24:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
|