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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450760
Gene symbol: Robo1
Gene name: roundabout guidance receptor 1
Probe: UI-M-AP0-abi-h-08-0-UI.s1
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Robo1 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Strong Non-Uniform Robo1-Shh E11.5_sagittal , Robo1-Shh E11.5_coronal Staining is strong in the MZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, PVH nucleus & AVH, moderate in the mam & supramam zones, weak in anterior & posterior IDs & either absent or ubiquitous in the VMH & ARH nuclei, premam zone & TT.
TS19: diencephalon lateral wall ventricular layer Ambiguous Robo1-Shh E11.5_sagittal , Robo1-Shh E11.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Strong Non-Uniform Robo1-Shh E11.5_sagittal , Robo1-Shh E11.5_coronal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex
TS19: telencephalon ventricular layer Ambiguous Robo1-Shh E11.5_sagittal , Robo1-Shh E11.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Robo1 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Robo1-Shh E12.5_coronal , Robo1-Shh E12.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Robo1-Shh E12.5_coronal , Robo1-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Robo1-Shh E12.5_coronal , Robo1-Shh E12.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Strong Non-Uniform Robo1-Shh E12.5_coronal , Robo1-Shh E12.5_sagittal There is strong staining in the mantle zone of the TT and mam zone and weak staining in the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS20: diencephalon ventricular layer Ambiguous Robo1-Shh E12.5_sagittal , Robo1-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Very strong Non-Uniform Robo1-Shh E12.5_sagittal , Robo1-Shh E12.5_coronal There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer Strong Non-Uniform Robo1-Shh E12.5_coronal , Robo1-Shh E12.5_sagittal There is strong staining in the mantle zone of the VMH nucleus and weak staining in the dorsal and ventral POAs. Staining is either absent or ubiquitous in the mantle zone of the PVH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Weak Non-Uniform Robo1-Shh E12.5_sagittal , Robo1-Shh E12.5_coronal There is weak staining in the ventricular zone of the anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and the AVH.
TS20: thalamus mantle layer Very strong Robo1-Shh E12.5_sagittal , Robo1-Shh E12.5_coronal There is very strong staining in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Ambiguous Robo1-Shh E12.5_coronal , Robo1-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Robo1 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Moderate Robo1-Shh E14.5_sagittal , Robo1-Shh E14.5_coronal There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous Robo1-Shh E14.5_coronal , Robo1-Shh E14.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Moderate Robo1-Shh E14.5_sagittal , Robo1-Shh E14.5_coronal There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Strong Non-Uniform Robo1-Shh E14.5_sagittal , Robo1-Shh E14.5_coronal There is strong staining in the mantle zone of the TT and the mam zone and weak staining in the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS22: diencephalon ventricular layer Ambiguous Robo1-Shh E14.5_coronal , Robo1-Shh E14.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Moderate Non-Uniform Robo1-Shh E14.5_sagittal , Robo1-Shh E14.5_coronal There is moderate staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Moderate Non-Uniform Robo1-Shh E14.5_sagittal , Robo1-Shh E14.5_coronal There is moderate staining in the MZ of the dorsal and ventral POAs and the AVH and weak staining in the PVH nucleus. Staining is either absent or ubiquitous in the MZ of the anterior and posterior IDs, the VMH and ARH nuclei and the AVH.
TS22: hypothalamus ventricular layer Weak Non-Uniform Robo1-Shh E14.5_coronal , Robo1-Shh E14.5_sagittal There is weak staining in the ventricular zone of the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs and the PVH, VMH and ARH nuclei.
TS22: thalamus mantle layer Strong Non-Uniform Robo1-Shh E14.5_coronal , Robo1-Shh E14.5_sagittal There is strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS22: thalamus ventricular layer Strong Robo1-Shh E14.5_sagittal , Robo1-Shh E14.5_coronal There is strong staining in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Robo1 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Strong Robo1-Shh E16.5_sagittal , Robo1-Shh E16.5_coronal There is strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous Robo1-Shh E16.5_sagittal , Robo1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Strong Robo1-Shh E16.5_sagittal , Robo1-Shh E16.5_coronal There is strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Weak Non-Uniform Robo1-Shh E16.5_coronal , Robo1-Shh E16.5_sagittal There is weak staining in the mantle zone of the mam and supramam zones. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS24: diencephalon ventricular layer Strong Non-Uniform Robo1-Shh E16.5_sagittal , Robo1-Shh E16.5_coronal There is strong staining in the ventricular zone of the premam and mam zones. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS24: ganglionic eminence Strong Non-Uniform Robo1-Shh E16.5_coronal , Robo1-Shh E16.5_sagittal There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer Moderate Non-Uniform Robo1-Shh E16.5_coronal , Robo1-Shh E16.5_sagittal There is moderate staining in the mantle zone of the dorsal and ventral POAs and weak staining in the AVH and the DAH. Staining is either absent or ubiquitous in the mantle zone of the PVH, SCH, DMH, VMH and ARH nuclei and the PH.
TS24: hypothalamus ventricular layer Strong Non-Uniform Robo1-Shh E16.5_sagittal , Robo1-Shh E16.5_coronal There is strong staining in the VZ of the VMH nucleus & the PH, moderate staining in the AVH, the DAH & DMH nucleus & weak staining in the PVH nucleus. Staining is either absent or ubiquitous in the VZ of the dorsal & ventral POAs & SCH & ARH nuclei.
TS24: thalamus mantle layer Ambiguous Robo1-Shh E16.5_coronal , Robo1-Shh E16.5_sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Strong Non-Uniform Robo1-Shh E16.5_coronal , Robo1-Shh E16.5_sagittal There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.


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last database update
07/29/2020
MGI 6.15
The Jackson Laboratory