Gene Expression Data
Assay Details
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Assay
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Reference:
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J:150715
Malcuit C, et al., Identification of novel oocyte and granulosa cell markers. Gene Expr Patterns. 2009 Sep;9(6):404-10
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4355534
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Gene symbol:
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Amfr
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Gene name:
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autocrine motility factor receptor
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Probe:
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Amfr probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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This data is summarized in Figure 3.
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Results
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Specimen
2A:
postnatal week 6
(more  )
Note: Primary follicle.
Specimen
2A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Primary follicle.
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Specimen
2B:
postnatal week 6
(more )
Note: Secondary/Tertiary follicle.
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Secondary/Tertiary follicle.
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Specimen
2C:
postnatal week 6
(more )
Note: Early Antral follicle.
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Early Antral follicle.
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Specimen
2D:
postnatal week 6
(more )
Note: Antral follicle.
Specimen
2D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Antral follicle.
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Specimen
2E:
postnatal week 6
(more )
Note: Antral follicle.
Specimen
2E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Antral follicle.
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Specimen
2F:
postnatal week 6
(more )
Note: Corpus Lutea.
Specimen
2F:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Corpus Lutea.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus luteum |
Present |
Non-Uniform |
2F
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Expression is present in some, but not all, cells.
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Specimen
2G:
postnatal week 6
(more )
Specimen
2G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
Regionally restricted |
2G
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Expression is present in all secondary, antral, and some tertiary oocytes; it is not present in any primary oocytes.
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| TS28: oocyte of secondary follicle |
Present |
Regionally restricted |
2G
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Expression is present in all secondary, antral, and some tertiary oocytes; it is not present in any primary oocytes.
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| TS28: oocyte of tertiary follicle |
Present |
Regionally restricted |
2G
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Expression is present in all secondary, antral, and some tertiary oocytes; it is not present in any primary oocytes.
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| TS28: oocyte of primary follicle |
Absent |
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2G
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Expression is present in all secondary, antral, and some tertiary oocytes; it is not present in any primary oocytes.
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| TS28: follicle cell of antral follicle |
Present |
Regionally restricted |
2G
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Expression is present in some secondary, tertiary, and antral granulosa cells; it is not present in primary granulosa cells.
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| TS28: follicle cell of secondary follicle |
Present |
Regionally restricted |
2G
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Expression is present in some secondary, tertiary, and antral granulosa cells; it is not present in primary granulosa cells.
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| TS28: follicle cell of tertiary follicle |
Present |
Regionally restricted |
2G
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Expression is present in some secondary, tertiary, and antral granulosa cells; it is not present in primary granulosa cells.
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| TS28: follicle cell of primary follicle |
Absent |
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2G
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Expression is present in some secondary, tertiary, and antral granulosa cells; it is not present in primary granulosa cells.
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| TS28: corpus luteum |
Present |
Non-Uniform |
2G
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Expression is present in some, but not all, cells.
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Analysis Tools