Probe:	mNFI-A cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS14: heart	Present
TS14: septum transversum	Present
TS14: future brain	Present			Expression is present in the anterior and posterior portions of the developing brain.

Structure	Level	Pattern	Image	Note
TS17: embryo mesenchyme	Present	Regionally restricted		Expression is present in mesenchyme surrounding the posterior cardinal and vitelline veins.
TS17: neural tube	Present	Regionally restricted		Expression is present in the ventral portion of the neural tube.
TS17: limb	Present	Regionally restricted		Expression is present in the distal margin of limb buds.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	3A	Expression is present in the neocortex.
TS19: brain ventricular layer	Present		3A
TS19: dorsal root ganglion	Present		3A
TS19: 1st branchial arch	Present	Regionally restricted	3A	Expression is present the more proximal mesenchyme of the mandibular arch.
TS19: lung	Present		3A
TS19: liver	Present		3A
TS19: gut mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS19: gonad	Strong
TS19: neural tube	Present	Regionally restricted		Expression is present in the ependymal layer of the neural tube.
TS19: limb mesenchyme	Present	Regionally restricted		Expression is present in distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Non-Uniform		Expression was the same as was described for E11.5.
TS20: skeletal muscle	Present	Regionally restricted		Expression is present in the developing skeletal muscles of the limbs and trunk.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	4A	Expression was the same as was described for E11.5.
TS22: limb musculature	Present		4A
TS22: skeletal muscle	Present	Regionally restricted	4A	Expression is present in the developing skeletal muscles of the trunk.
TS22: head mesenchyme	Present	Regionally restricted	4A	Expression is present in mesenchyme around nasal sinuses.
TS22: limb interdigital region	Present		4A
TS22: gonad	Present		4A
TS22: neocortex	Very strong		4A
TS22: cranial ganglion	Present		4A

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS23: skin	Strong	Regionally restricted		Expression is present in adipocytes of brown fat pads.
TS23: salivary gland	Present

Structure	Level	Pattern	Image	Note
TS24: brown fat	Strong	Regionally restricted	5A	Expression is present in adipocytes of brown fat pads.
TS24: salivary gland	Present		5A
TS24: pons	Present		5A	Expression is present in pontine nuclei.
TS24: cerebral cortex	Strong		5A
TS24: skin	Present	Regionally restricted	5A	Expression is present around the whisker follicles.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	6A	Expression is predominantly in the white matter, suggesting it is expressed by glia.
TS28: dentate gyrus	Present		6A	Expression levels in the dentate gyrus are higher than in the horn of Ammon cells.
TS28: hippocampus	Present		6A

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present