Gene Expression Data
Assay Details
Assay
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Reference:
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J:47185
Kamata T, et al., Isolation and characterization of a bovine neural specific protein (CRMP-2) cDNA homologous to unc-33, a C. elegans gene implicated in axonal outgrowth and guidance. Brain Res Mol Brain Res. 1998 Mar 1;54(2):219-36
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3813919
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Gene symbol:
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Dpysl2
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Gene name:
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dihydropyrimidinase-like 2
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Antibody:
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Anti-CRMP-2 #N17
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixative: 5% acetic acid, 95% ethanol.
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Results
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Specimen
6A:
embryonic day 16.5
(more )
Specimen
6A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
6B:
embryonic day 16.5
(more )
Specimen
6B:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Present |
Regionally restricted |
6B
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Expression was detected in the inner layer of the differentiating cells and growing axons of the ganglion cells, but not in the outer layer of the dividing cells.
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Specimen
6C:
embryonic day 16.5
(more )
Specimen
6C:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Present |
Regionally restricted |
6C
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Expression was detected in the inner layer of the differentiating cells and growing axons of the ganglion cells, but not in the outer layer of the dividing cells.
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Specimen
6D:
embryonic day 16.5
(more )
Specimen
6D:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
6E:
embryonic day 16.5
(more )
Specimen
6E:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: olfactory epithelium |
Present |
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6E
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Expression was detected in the dendrites, cell bodies and axons of the primary olfactory neurons.
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Specimen
6F:
embryonic day 16.5
(more )
Specimen
6F:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
6G:
embryonic day 16.5
(more )
Specimen
6G:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: myenteric nerve plexus |
Present |
Regionally restricted |
6G
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Expression was detected in the myenteric plexus in the small intestine.
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Specimen
7A,7D:
embryonic day 16.5
(more )
Note: Fig. 7D is a higher magnification image of 7A. Coronal section at the level of the optic chiasma.
Specimen
7A,7D:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fig. 7D is a higher magnification image of 7A. Coronal section at the level of the optic chiasma.
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Structure |
Level |
Pattern |
Image |
Note |
TS24: brain |
Strong |
Ubiquitous |
7A
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TS24: brain ventricular layer |
Absent |
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7A
,
7D
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No expression was detected in the differentiated ependymal cells and choroidal plexus cells.
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TS24: choroid plexus |
Absent |
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7A
,
7D
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No expression was detected in the differentiated ependymal cells and choroidal plexus cells.
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Specimen
7B,7E:
embryonic day 16.5
(more )
Note: Fig. 7E is a higher magnification image of 7B. Coronal section at the level of the anterior pituitary.
Specimen
7B,7E:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fig. 7E is a higher magnification image of 7B. Coronal section at the level of the anterior pituitary.
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Structure |
Level |
Pattern |
Image |
Note |
TS24: brain |
Strong |
Ubiquitous |
7B
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TS24: cerebral cortex |
Present |
Regionally restricted |
7B
,
7E
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Expression was detected in the neuroepithelial cell zone. Very low levels of expression were seen in radial processes extending to the ventricular surface. High levels were detected in the upper differentiating zones.
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TS24: cortical plate |
Present |
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7E
,
7B
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Expression was highest in the apical dendrites growing to the outer surface from the neuronal cell bodies.
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TS24: cortical subplate |
Present |
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7B
,
7E
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Expression was high in the cytoplasm of the cell bodies and possibly the terminals of the contacting axons.
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TS24: cerebral cortex intermediate zone |
Present |
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7B
,
7E
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Expression was predominantly detected in the axons.
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TS24: cerebral cortex subventricular zone |
Present |
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7B
,
7E
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Expression was predominantly detected in the axons.
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Specimen
7C,7F:
embryonic day 16.5
(more )
Note: Fig. 7F is a higher magnification image of 7C. Coronal section at the level of the mammilary body.
Specimen
7C,7F:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Fig. 7F is a higher magnification image of 7C. Coronal section at the level of the mammilary body.
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Structure |
Level |
Pattern |
Image |
Note |
TS24: brain |
Strong |
Ubiquitous |
7C
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TS24: pons |
Present |
Regionally restricted |
7F
,
7C
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Expression was detected in the transverse fibers of the pons.
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Specimen
8A:
postnatal day 60
(more )
Specimen
8A:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Present |
Ubiquitous |
8A
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Specimen
8B:
postnatal day 60
(more )
Specimen
8B:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8C:
postnatal day 60
(more )
Specimen
8C:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8D:
postnatal day 60
(more )
Specimen
8D:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8E:
postnatal day 60
(more )
Specimen
8E:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
8F:
postnatal day 60
(more )
Specimen
8F:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS28: CA1 pyramidal cell layer |
Present |
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8F
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The dendrites and cell bodies of the pyramidal cells in the CA1 field of the hippocampus were stained.
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