Adcy9 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:128545 Visel A, et al., Comprehensive analysis of the expression patterns of the adenylate cyclase gene family in the developing and adult mouse brain. J Comp Neurol. 2006 Jun 10;496(5):684-97
Assay type:	RNA in situ
MGI Accession ID:	MGI:3768402
Gene symbol:	Adcy9
Gene name:	adenylate cyclase 9

Probe:	MH855 probe 829
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Results

Specimen Table 2-P7: postnatal day 7 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb mitral cell layer	Strong
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: main olfactory bulb granule cell layer	Strong
TS28: cerebral cortex	Weak
TS28: hippocampus CA1	Strong
TS28: hippocampus CA2	Strong
TS28: hippocampus CA3	Strong
TS28: dentate gyrus	Moderate
TS28: corpus striatum	Weak
TS28: telencephalon septum	Absent
TS28: amygdala	Weak
TS28: thalamus	Moderate
TS28: hypothalamus	Weak
TS28: pretectum	Weak
TS28: superior colliculus	Weak
TS28: inferior colliculus	Weak
TS28: midbrain tegmentum	Weak
TS28: cerebellum molecular layer	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum granule cell layer	Absent
TS28: pons	Moderate
TS28: medulla oblongata	Moderate

BA107 ©

MH1909 ©

Specimen Table 2-P56: postnatal day 56 (more

)

Structure	Level	Image
TS28: main olfactory bulb glomerular layer	Moderate	MH1909 , BA107
TS28: main olfactory bulb external plexiform layer	Absent	MH1909 , BA107
TS28: main olfactory bulb mitral cell layer	Strong	MH1909 , BA107
TS28: main olfactory bulb internal plexiform layer	Absent	BA107 , MH1909
TS28: main olfactory bulb granule cell layer	Strong	MH1909 , BA107
TS28: cerebral cortex	Moderate	BA107 , MH1909
TS28: hippocampus CA1	Strong	BA107 , MH1909
TS28: hippocampus CA2	Strong	MH1909 , BA107
TS28: hippocampus CA3	Strong	BA107 , MH1909
TS28: dentate gyrus	Strong	BA107 , MH1909
TS28: corpus striatum	Weak	BA107 , MH1909
TS28: telencephalon septum	Weak	BA107 , MH1909
TS28: amygdala	Moderate	MH1909 , BA107
TS28: thalamus	Moderate	BA107 , MH1909
TS28: hypothalamus	Weak	BA107 , MH1909
TS28: pretectum	Weak	BA107 , MH1909
TS28: superior colliculus	Weak	BA107 , MH1909
TS28: inferior colliculus	Weak	BA107 , MH1909
TS28: midbrain tegmentum	Weak	MH1909 , BA107
TS28: cerebellum molecular layer	Absent	MH1909 , BA107
TS28: cerebellum Purkinje cell layer	Weak	MH1909 , BA107
TS28: cerebellum granule cell layer	Weak	BA107 , MH1909
TS28: pons	Moderate	MH1909 , BA107
TS28: medulla oblongata	Moderate	BA107 , MH1909

1F ©

Specimen 1F: postnatal day 56 (more

)
Note: This is a sagittal section.

Structure	Level	Image
TS28: main olfactory bulb glomerular layer	Strong	1F
TS28: main olfactory bulb mitral cell layer	Strong	1F
TS28: main olfactory bulb granule cell layer	Present	1F

Specimen Not shown: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Absent
TS22: main olfactory bulb	Absent

2G ©

Specimen 2G: postnatal day 56 (more

)
Note: This is a coronal section.

Structure	Level	Image	Note
TS28: cortical layer II	Present	2G	Expression is present in layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer III	Present	2G	Expression is present in layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer VI	Present	2G	This is one of layers with the highest expression levels.
TS28: cortical layer V	Present	2G

3M ©

Specimen 3M: postnatal day 7 (more

)
Note: This is a sagittal section.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3M

3N ©

Specimen 3N: postnatal day 56 (more

)
Note: This is a coronal section.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3N

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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