Probe:	MH852 probe 826
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb mitral cell layer	Moderate
TS28: main olfactory bulb internal plexiform layer	Absent
TS28: main olfactory bulb granule cell layer	Moderate
TS28: cerebral cortex	Moderate
TS28: hippocampus CA1	Moderate
TS28: hippocampus CA2	Moderate
TS28: hippocampus CA3	Moderate
TS28: dentate gyrus	Moderate
TS28: corpus striatum	Moderate	Single cells
TS28: telencephalon septum	Weak
TS28: amygdala	Moderate
TS28: thalamus	Moderate
TS28: hypothalamus	Moderate
TS28: pretectum	Weak
TS28: superior colliculus	Weak
TS28: inferior colliculus	Weak
TS28: midbrain tegmentum	Weak
TS28: cerebellum molecular layer	Absent
TS28: cerebellum Purkinje cell layer	Absent
TS28: cerebellum granule cell layer	Absent
TS28: pons	Weak
TS28: medulla oblongata	Weak

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Moderate		MH1907 , BA104
TS28: main olfactory bulb external plexiform layer	Absent		MH1907 , BA104
TS28: main olfactory bulb mitral cell layer	Moderate		MH1907 , BA104
TS28: main olfactory bulb internal plexiform layer	Absent		BA104 , MH1907
TS28: main olfactory bulb granule cell layer	Moderate		BA104 , MH1907
TS28: cerebral cortex	Moderate		BA104 , MH1907
TS28: hippocampus CA1	Weak		MH1907 , BA104
TS28: hippocampus CA2	Moderate		MH1907 , BA104
TS28: hippocampus CA3	Weak		MH1907 , BA104
TS28: dentate gyrus	Moderate		MH1907 , BA104
TS28: corpus striatum	Moderate	Single cells	BA104 , MH1907
TS28: telencephalon septum	Weak		MH1907 , BA104
TS28: amygdala	Moderate		MH1907 , BA104
TS28: thalamus	Weak		BA104 , MH1907
TS28: hypothalamus	Weak		MH1907 , BA104
TS28: pretectum	Weak		BA104 , MH1907
TS28: superior colliculus	Weak		BA104 , MH1907
TS28: inferior colliculus	Weak		MH1907 , BA104
TS28: midbrain tegmentum	Weak		MH1907 , BA104
TS28: cerebellum molecular layer	Absent		MH1907 , BA104
TS28: cerebellum Purkinje cell layer	Absent		MH1907 , BA104
TS28: cerebellum granule cell layer	Weak		MH1907 , BA104
TS28: pons	Weak		BA104 , MH1907
TS28: medulla oblongata	Weak		MH1907 , BA104

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		1D
TS28: main olfactory bulb mitral cell layer	Strong		1D

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Absent
TS22: main olfactory bulb	Absent
TS22: stomach	Strong	Regionally restricted		There is strong expression throughout the mucosal lining of the stomach.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		2E	Expression is present in layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer III	Present		2E	Expression is present in layers 2/3. This is one of layers with the highest expression levels.
TS28: cortical layer V	Present		2E	This is one of layers with the highest expression levels.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3I

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3J

Structure	Level	Pattern	Image	Note
TS22: tectum	Present	Regionally restricted	6D	Expression is present in the developing tectum at the midline, as well as in more lateral sections. It is found in the mantle layer and in the neuroepithelium.