Gene Expression Data
Assay Details
Assay
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Reference:
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J:63183
Kramer PR, et al., Ectopic expression of luteinizing hormone-releasing hormone and peripherin in the respiratory epithelium of mice lacking transcription factor AP-2alpha. Mech Dev. 2000 Jun;94(1-2):79-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3043831
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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anti-pro-LHRH antibody (SW-1)
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Detection system:
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horseradish peroxidase
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Assay notes:
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The authors did not specify which antibody was used to obtain these data; it is possible MGI:5467573 was used instead.
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Results
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Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Present |
Single cells |
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Expression is present in cells in the nasal region.
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Specimen
Not shown:
(close )
Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: head |
Present |
Single cells |
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Expression is present in cells in the nasal region. Expression in the mutants is the same as in wild type.
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Specimen
3C:
embryonic day 13.5
(more )
Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: forebrain |
Present |
Regionally restricted |
3C
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
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TS21: nasal cavity |
Present |
Regionally restricted |
3C
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
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TS21: nasal septum |
Present |
Regionally restricted |
3C
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
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Specimen
3D:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: forebrain |
Present |
Regionally restricted |
3D
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain. Expression in this mutant is the same as in wild-type embryos.
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TS21: nasal cavity |
Present |
Regionally restricted |
3D
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain. Expression in this mutant is the same as in wild-type embryos.
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TS21: nasal septum |
Present |
Regionally restricted |
3D
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain. Expression in this mutant is the same as in wild-type embryos.
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Specimen
3E:
embryonic day 14.5
(more )
Specimen
3E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forebrain |
Present |
Regionally restricted |
3E
|
Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
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TS22: nasal cavity |
Present |
Regionally restricted |
3E
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
|
TS22: nasal septum |
Present |
Regionally restricted |
3E
|
Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
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Specimen
3F:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Fresh Frozen
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forebrain |
Present |
Regionally restricted |
3F
|
Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
|
TS22: nasal cavity |
Present |
Regionally restricted |
3F
|
Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
|
TS22: nasal septum |
Present |
Regionally restricted |
3F
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Expression is in migrating cells leaving the olfactory pit, migrating across the nasal septum into the forebrain.
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TS22: nasal cavity epithelium |
Present |
Regionally restricted |
3F
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A small population of expressing cells was detected in the rostral epithelium.
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Specimen
4B:
(close )
Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Double labeled: blue/black - Gnrh1; brown - Ebf1 (Olf-1; assay MGI:3043835).
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Specimen
4C:
(close )
Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: nose |
Present |
Regionally restricted |
4C
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Expression is adjacent to the respiratory epithelium.
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Specimen
4F:
(close )
Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: nose |
Present |
Regionally restricted |
4F
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Expression is adjacent to the respiratory epithelium.
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Specimen
5A,B:
(close )
Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Double labeled: blue/black - Gnrh1; brown - Prph (assay MGI:3043830).
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Specimen
5C,D:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
Note:
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Double labeled: blue/black - Gnrh1; brown - Ncam1 (assay MGI:3043836).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: nasal septum |
Present |
Regionally restricted |
5D
,
5C
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Expression is in a cluster of cells.
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Specimen
7A,B:
(close )
Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Double labeled: blue/black - Gnrh1; brown - Mki67 (assay MGI:3043837).
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Specimen
7C:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue/black - Gnrh1; brown - Mki67 (assay MGI:3043837).
|
Specimen
7E:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
|
Tfap2atm1Jae/Tfap2atm1Jae
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue/black - Gnrh1; brown - Mki67 (assay MGI:3043837).
|
|