Probe:	PlexinA2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: spinal cord roof plate	Strong		1A
TS20: spinal cord	Present	Regionally restricted	1A	Expression throughout the ventral neural tube excluding the ventral horns.
TS20: dorsal root ganglion	Present		1A

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	1B	Expression adjacent to the pulmonary trunk and aortic arch arteries.
TS20: arch of aorta	Present		1B

Structure	Level	Pattern	Image	Note
TS20: migrating neural crest	Present	Regionally restricted	1C	The authors state that a column of expressing cells is seen extending into the outflow tract endocardial cushions consistent with expression by migrating cardiac neural crest.
TS20: outflow tract	Present	Regionally restricted	1C	The authors state that a column of expressing cells is seen extending into the outflow tract endocardial cushions consistent with expression by migrating cardiac neural crest.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present		4A	Expression in the neural folds.
TS13: neural tube	Present		4A	Expression in the neural folds.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	4B	Expression in the dorsal neural tube at the level of the first three somites, the region from which cardiac neural crest emerges.

Structure	Level	Pattern	Image	Note
TS17: neural tube roof plate	Present		4C

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		4D	Expression throughout the pharyngeal arches.
TS17: arterial system	Present		4D	Expression surrounding the aortic arch arteries.

Structure	Level	Pattern	Image	Note
TS17: bulbus cordis rostral half	Present		4E

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Present	Regionally restricted	4F	Expressing cells surrounding the aorta and pulmonary trunk.
TS19: outflow tract	Present	Regionally restricted	4F	Expression extedending into the outflow tract mesenchyme.
TS19: left ventricle cardiac muscle	Present		4F

Structure	Level	Pattern	Image	Note
TS20: spinal cord roof plate	Present		5A
TS20: spinal cord	Present	Regionally restricted	5A	Expression excludes the ventral horns.
TS20: dorsal root ganglion	Present		5A
TS20: outflow tract	Present	Regionally restricted	5A	Expression around the aorta.
TS20: arterial system	Present	Regionally restricted	5A	Expression in the ductus arteriosus.
TS20: trunk mesenchyme	Present	Regionally restricted	5A	Expression surrounding the ductus arteriosus and dorsal aorta.

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Present	Regionally restricted	5B	Expression in a population of cells in the condensed mesenchyme of the outflow tract where neural crest cells reside.
TS20: spinal cord roof plate	Present		5B
TS20: trunk mesenchyme	Present	Regionally restricted	5B	Expression surrounding the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	5C	Expression in the smooth muscle of the intestines.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		5E	Expression in the bronchial smooth muscle.

Structure	Level	Pattern	Image	Note
TS20: left ventricle cardiac muscle	Weak		7B
TS20: heart	Present	Regionally restricted	7B	A localized collection of expressing cells in the condensed mesnehcyme.

Structure	Level	Pattern	Image	Note
TS20: left ventricle cardiac muscle	Strong		7E
TS20: endocardial cushion	Weak		7E