Serpina1em#Chmu
Endonuclease-mediated Allele Detail
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Symbol: |
Serpina1em#Chmu |
Name: |
serine (or cysteine) peptidase inhibitor, clade A, member 1 cluster; endonuclease-mediated mutation, Christian Mueller |
MGI ID: |
MGI:6358591 |
Gene: |
Serpina1 Location: unknown Genetic Position: Chr12, cytoband E-F
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Allele Type: |
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Endonuclease-mediated (Null/knockout) |
Mutation: |
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Intergenic deletion
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Serpina1em#Chmu involves 4 genes/genome features (Serpina1b, Serpina1d, Serpina1a ...)
View all
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Mutation details: CRISPR/Cas9 technology is used to disrupt five highly homologous gene paralogs through the targeting of exon 2 using a combination of four gRNAs. The population analyzed for phenotype contained individuals with alleles from the 3 lines (7A, 24B, 31C) and includes Serpina1em1Chmu, Serpina1em2Chmu, and Serpina1em2Chmu. The pound symbol (#) is used when no line is specified or lines are pooled. Western blot and immunohistochemistry confirmed absence of the alpha1-antitrypsin protein in serum and liver tissue, respectively.
(J:259615)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Serpina1 Mutation: |
1 strain or line available
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Original: |
J:259615 Borel F, et al., Editing out five Serpina1 paralogs to create a mouse model of genetic emphysema. Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2788-2793 |
All: |
2 reference(s) |
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