Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Plp1-Gal3st1)2645Gie
Name:	transgene insertion 2645, Volkmar Gieselmann
MGI ID:	MGI:6118919
Synonyms:	PLP-Gal3stl tg
Transgene:	Tg(Plp1-Gal3st1)2645Gie  Location: unknown
Alliance:	Tg(Plp1-Gal3st1)2645Gie page

Strain of Origin:

(C57BL/6 x CBA)F2

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(Plp1-Gal3st1)2645Gie expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Gal3st1 (MGI:1858277)   Mutation details: The transgenic construct contains (from 5' to 3') a ~12.7 kbp promoter/enhancer sequence from the mouse proteolipid protein (myelin) 1 locus, a ~1.3 kbp cDNA sequence encoding mouse galactose-3-O-sulfotransferase 1 and an SV40 polyA sequence. The PLP fragment includes its 5' flanking DNA, exon 1, intron 1 and partial exon 2 sequences, with mutations introduced into the exon 1 ATG start codon and an exon 2 ATG (to allow protein translation to start at the appropriate ATG of the inserted CST cDNA - while keeping PLP exon/intron consensus sequences that are necessary for proper splicing), as well as deletion of the first 13 amino acids of exon 2. Female founder tg2645 has a 200-fold increased Gal3st1 enzyme activity in brain homogenates (4-6 months old) compared to wildtype controls. (J:124942)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:124942 Ramakrishnan H, et al., Increasing sulfatide synthesis in myelin-forming cells of arylsulfatase A-deficient mice causes demyelination and neurological symptoms reminiscent of human metachromatic leukodystrophy. J Neurosci. 2007 Aug 29;27(35):9482-90
All:	2 reference(s)