Col1a1tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu
Targeted Allele Detail
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| Symbol: |
Col1a1tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu |
| Name: |
collagen, type I, alpha 1; targeted mutation 4, Yasuhiro Yamada |
| MGI ID: |
MGI:5621181 |
| Synonyms: |
Col1a1::tetO-OSKM-ires-mCherry, Col1a1tm4(tetO-OSKM,-mCherry)Yasu, OSKM-inducible |
| Gene: |
Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
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| Alliance: |
Col1a1tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu page
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| Allele Type: |
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Targeted (Inducible, Inserted expressed sequence, Reporter) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Col1a1tm4(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Yasu expresses
4 genes
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Mutation details: The tetOP-OKSM "flip-in plasmid" contained a splice acceptor-double polyA sequence and the tetracycline responsive element (TRE, tetOP, or tetO) upstream of the four mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Klf4 (Kruppel-like factor 4 (gut)), Sox2 (SRY-box containing gene 2), and c-Myc (Myc; myelocytomatosis oncogene) separated by three different 2A sequences that mediate ribosomal skipping (F2A [from foot-and-mouth disease virus], IRES [internal ribosome entry site], and E2A [from equine rhinitis A virus], respectively) and followed by an IRES-mCherry.
(J:212039)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Col1a1 Mutation: |
160 strains or lines available
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| Original: |
J:212039 Ohnishi K, et al., Premature termination of reprogramming in vivo leads to cancer development through altered epigenetic regulation. Cell. 2014 Feb 13;156(4):663-77 |
| All: |
4 reference(s) |
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Analysis Tools
