Notch2tm3.1Rko
Targeted Allele Detail
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| Symbol: |
Notch2tm3.1Rko |
| Name: |
notch 2; targeted mutation 3.1, Raphael Kopan |
| MGI ID: |
MGI:5523517 |
| Synonyms: |
N21, N221 |
| Gene: |
Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
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| Alliance: |
Notch2tm3.1Rko page
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| Allele Type: |
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Targeted (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Notch2tm3.1Rko expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Notch1 (MGI:97363) |
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Mutation details: A targeting vector constructed by BAC recombineering methods was used to swap the Notch2 intracellular domain (N2ICD) with the ICD of Notch1 (N1ICD) encoding amino acids 1,750 to 2,531, by homologous recombination in B6-derived ES cells. The 3' UTR of Notch2 was not switched so that mRNA stability could be retained. After establishment of germline transmission of this allele, the frt-flanked neo marker that had been introduced as part of the targeting vector was removed by crossing mice to flippase deleter mice.
(J:198632)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Notch2 Mutation: |
97 strains or lines available
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| Original: |
J:198632 Liu Z, et al., The Extracellular Domain of Notch2 Increases Its Cell-Surface Abundance and Ligand Responsiveness during Kidney Development. Dev Cell. 2013 Jun 24;25(6):585-98 |
| All: |
2 reference(s) |
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Analysis Tools
