Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Nr6a1tm1c(EUCOMM)Wtsi
Name:	nuclear receptor subfamily 6, group A, member 1; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID:	MGI:5510737
Synonyms:	Gcnffl
Gene:	Nr6a1  Location: Chr2:38613381-38816473 bp, - strand  Genetic Position: Chr2, 24.42 cM
Alliance:	Nr6a1tm1c(EUCOMM)Wtsi page
IMPC:	Nr6a1 gene page

Mutant Cell Lines:	EPD0105_6_D03, EPD0105_6_D04
Germline Transmission:	Earliest citation of germline transmission: J:204241
Parent Cell Line:	JM8.F6 (ES Cell)
Strain of Origin:	C57BL/6N
Project Collection:	EUCOMM

Allele Type:    Targeted (Conditional ready) Mutation:    Insertion     Vector: L1L2_Bact_P   Mutation details: The L1L2_Bact_P cassette was inserted at position 38632589 of Chromosome 2 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 38633441. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Flp-mediated recombination removed the reporter and neomycin resistance cassette and left exon 7 floxed. (J:173534, J:204241)

Generation of the Nr6a1tm1a(EUCOMM)Wtsi, Nr6a1tm1c(EUCOMM)Wtsi, and Nr6a1tm1d(EUCOMM)Wtsi alleles

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nr6a1 Mutation:	145 strains or lines available

Original:	J:173534 Skarnes WC, et al., A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 2011 Jun 16;474(7351):337-42
All:	2 reference(s)