Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Nrastm2.1Emfu
Name:	neuroblastoma ras oncogene; targeted mutation 2.1, Ernst-Martin Fuchtbauer
MGI ID:	MGI:5502420
Synonyms:	LTR9NAS
Gene:	Nras  Location: Chr3:102965643-102975230 bp, + strand  Genetic Position: Chr3, 45.25 cM
Alliance:	Nrastm2.1Emfu page

Germline Transmission:	Earliest citation of germline transmission: J:199340
Parent Cell Line:	CJ7 (ES Cell)
Strain of Origin:	129S1/Sv-Oca2+ Tyr+ Kitl+

Allele Type:    Targeted (Not Applicable) Mutation:    Insertion   Mutation details: A floxed PGK/Tn5 neomycin resistance (neo) cassette and the Akv1-99 murine leukemia virus long terminal repeat were inserted into intron 1 via homologous recombination. The viral long terminal repeat was inserted at the proviral integration site 9 in the anti-sense direction. Cre-mediated recombination removed the neo cassette. Western blot analysis confirmed increased protein expression in the spleen and thymus. (J:199340)

Schematic for the generation of the Nrastm1.1Emfu, Nrastm2.1Emfu, Nrastm3Emfu, and Nrastm4Emfu alleles

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nras Mutation:	44 strains or lines available

Original:	J:199340 Ballarin-Gonzalez B, et al., Deregulated Nras expression in knock-in animals harboring a gammaretroviral long terminal repeat at the Nras/Csde1 locus. PLoS One. 2013;8(2):e56029
All:	1 reference(s)