Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae
Name:	collagen, type I, alpha 1; targeted mutation 7, Rudolf Jaenisch
MGI ID:	MGI:5007779
Synonyms:	TetOP-H2B-GFP
Gene:	Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae page

Germline Transmission:	Earliest citation of germline transmission: J:172421
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Humanized sequence, Inducible, Inserted expressed sequence, RMCE-ready) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae expresses 1 gene Knock-in expresses: Organism Expressed Gene Homolog in Mouse Note human H2BC11 (8970) H2bc21  (MGI:2448415)   Mutation details: RMCE on KH2 cells inserted a cassette that contains a cDNA encoding human H2B (HIST1H2BJ) tagged at its carboxyl terminus with DNA encoding codon-optimized enhanced GFP under the transcriptional control of a tetracycline-dependent minimal CMV promoter into the 3'UTR. (J:172421)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Original:	J:172421 Foudi A, et al., Analysis of histone 2B-GFP retention reveals slowly cycling hematopoietic stem cells. Nat Biotechnol. 2009 Jan;27(1):84-90
All:	40 reference(s)